文献类型：期刊文献

英文题名：Selection and Validation of Reference Genes for Quantitative RT-PCR Analysis in Corylus heterophylla Fisch. x Corylus avellana L.

作者：Hou, Sihao[1,2,3] Zhao, Tiantian[1,2,3] Yang, Dan[1,2,3] Li, Qing[1,2,3] Liang, Lisong[1,2,3] Wang, Guixi[1,2,3] Ma, Qinghua[1,2,3]

第一作者：Hou, Sihao

通信作者：Ma, QH[1];Ma, QH[2];Ma, QH[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[2]State Forestry & Grassland Adm, Hazelnut Engn & Tech Res Ctr, Beijing 100091, Peoples R China;[3]State Forestry & Grassland Adm, Natl Hazelnut Ind Innovat Alliance, Beijing 100091, Peoples R China

年份：2021

卷号：10

期号：1

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000610682900001)】；

基金：This research was funded by the Special Investigation on Basic Resources of Scienceand Technology (GrantNo. 2019FY100801-03) and the Special Fund for Basic Scientific Research Business of Central Public Research Institutes of the Chinese Academy of Forestry, China (CAFYBB2017ZA004-9).

语种：英文

外文关键词：Ping'ou hybrid hazelnut (C. heterophylla Fisch. x C. avellana L.); real-time quantitative PCR; reference gene; stability of gene expression; self-incompatibility

摘要：(1) Background: the species of Corylus have sporophytic type of self-incompatibility. Several genes related to recognition reaction between pollen and stigma have been identified in hazelnuts. To better understand the self-incompatibility (SI) response, we screened the suitable reference genes by using quantitative real-time reverse transcription PCR (qRT-PCR) analysis in hazelnut for the first time. (2) Methods: the major cultivar "Dawei" was used as material. A total of 12 candidate genes were identified and their expression profiles were compared among different tissues and in response to various treatments (different times after self- and cross-pollination) by RT-qPCR. The expression stability of these 12 candidate reference genes was evaluated using geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder programs. (3) Results: the comprehensive ranking of RefFinder indicated that ChaActin, VvActin, ChaUBQ14, and ChaEF1-alpha were the most suitable reference genes. According to the stability analysis of 12 candidate reference genes for each sample group based on four software packages, ChaActin and ChaEF1-alpha were most stable in different times after self-pollination and 4 h after self- and cross-pollination, respectively. To further validate the suitability of the reference genes identified in this study, CavPrx, which the expression profiles in Corylus have been reported, was quantified by using ChaActin and ChaEF1-alpha as reference genes. (4) Conclusions: our study of reference genes selection in hazelnut shows that the two reference genes, ChaActin and ChaEF1-alpha, are suitable for the evaluation of gene expression, and can be used for the analysis of pollen-pistil interaction in Corylus. The results supply a reliable foundation for accurate gene quantifications in Corylus species, which will facilitate the studies related to the reproductive biology in Corylus.