文献类型：期刊文献

英文题名：Identification and comparative profiling of microRNAs in wild-type Xanthoceras sorbifolia and its double flower mutant

作者：Ao, Yan[1,2,3,4] Wang, Yanwei[1] Chen, Lei[1] Wang, Tao[5] Yu, Haiyan[5] Zhang, Zhixiang[1,4]

第一作者：Ao, Yan

通信作者：Zhang, ZX[1]

机构：[1]Beijing Forestry Univ, Natl Engn Lab Tree Breeding, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ,Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[2]Beijing Forestry Univ, Acad Forestry, Beijing 100083, Peoples R China;[3]Natl Energy R&D Ctr Nonfood Biomass, Beijing 100083, Peoples R China;[4]Beijing Forestry Univ, Lab Systemat Evolut & Biogeog Woody Plants, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2012

卷号：34

期号：5

起止页码：561-568

外文期刊名：GENES & GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000310539900012)】；

基金：This research was financially supported by the Fundamental Research Funds for the Central Universities (NO. BLX2012035), Chinese Key Technology Research and Development Program of the Eleventh Five-year Plan (2006BAD18B0101, 2006BAD07A00), Chinese Key Technology Research and Development Program of the Twelfth Five-year Plan (2011BAD22B08), and the Forestry Public Benefit Research Program (201004001).

语种：英文

外文关键词：Simple flower; Double flower; MicroRNA; qRT-PCR; Xanthoceras sorbifolia

摘要：MicroRNAs are small, non-coding RNAs that regulate gene expression post-transcriptionally and have crucial roles in plant biological and metabolic processes. To date, there has been no report concerning miRNAs in Xanthoceras sorbifolia, one of the most important woody energy plants for biofuel production. To study the roles of miRNAs during floral organ differentiation and development in X. sorbifolia, two small RNA libraries were constructed from the flower buds of simple flowers (wild-type) and double flowers (mutant). Each library was sequenced individually and generated over 33 million raw reads in total. Forty-three conserved miRNAs belonging to 30 families and 10 novel miRNAs were identified. The expressions of a selected group of conserved and novel miRNAs were validated by qRT-PCR. Target gene prediction identified 230 target genes for most of the conserved and novel miRNAs. Fifteen miRNAs were demonstrated to be differentially expressed between wild-type and mutant. This is the first study to identify conserved and novel miRNAs and profile their expression changes during floral organ differentiation and development in X. sorbifolia.