文献类型：期刊文献

英文题名：Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

作者：Zhang, Shou-gong[1] Han, Su-ying[2] Yang, Wen-hua[1] Wei, Hua-li[1] Zhang, Ming[3] Qi, Li-wang[1]

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Cell Biol Lab, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab, Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[3]Jilin Acad Agr Sci, Ctr Biotechnol, Changchun 130033, Peoples R China

年份：2010

卷号：100

期号：1

起止页码：21-29

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000272905500003)】；

基金：This work was supported by the National High Technology Research and Development Program of China (Grant Nos. 2006AA100109, 2007AA021403, 2007AA10Z182, 2008AA10Z126 and 2007AA021501), the National Natural Science Foundation of China (Grant Nos. 30571517, 30830086), the National "948" Program (No. 2007-4-03, 2006-4-C01, 2006-4-C02), the National Basic Research Program of China (Grant Nos. 2009CB119106, 2009CB119107).

语种：英文

外文关键词：Somatic embryogenesis; Hydrogen peroxide; Catalase; Superoxide dismutase; Ascorbate peroxidase; L. leptolepis

摘要：Hydrogen peroxide (H2O2) content and transcript levels of genes encoding superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes were investigated during different stages of somatic embryogenesis in Larix leptolepis. H2O2 content was lowest on day 0 when embryogenic callus was incubated on Murashige and Skoog (MS) medium supplemented with polyethylene glycol (PEG). This content began to increase when callus was transferred to the same medium but containing abscisic acid (ABA), and reached a peak at day 3 following incubation. The level of H2O2 dropped from day 7 to day 10, peaked at day 21, then dropped again at day 24 and day 35, but increased when somatic embryos reached maturity at day 45. Transcript levels of SOD, CAT, and APX were lowest when somatic embryos were cultured on callus induction medium without ABA. When calli were transferred onto somatic embryo maturation medium, expression patterns of SOD, CAT, and APX varied, and transcript levels at all stages were higher than those at d 0. SOD expression was highest at day 3. Whereas, CAT expression levels were low during early stages of somatic embryogenesis, but increased at day 21, declined at days 24 and 35, and then began to increase again at day 45. APX gene expression patterns were highest at days 3, 21, and 45. These results suggested that ABA was essential for promoting somatic embryogenesis of L. leptolepis. Moreover, ABA induced production of H2O2 and other active oxygen species (AOS), and mediated CAT, SOD, and APX gene expression in somatic embryogenesis of L. leptolepis.