文献类型：期刊文献

中文题名：韩国、美国和中国栗疫病菌的遗传变异分析

英文题名：Genetic Variation of Strains of Cryphonectria parasitica from Korea,USA and China

作者：朴春根[1] Kim Kyung-hee[2] Lee Sang-hyun[2] Lee Seung-kyu[2] Moon Byung-ju[3]

第一作者：朴春根

机构：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室;[2]韩国国立山林科学院;[3]东亚大学

年份：2007

卷号：20

期号：2

起止页码：283-286

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：韩国科学财团(KOSEF)Brain Pool Program的资助

语种：中文

中文关键词：栗疫病;寄生隐丛赤壳;栗树;遗传变异

外文关键词：chestnut blight ; Cryphonectria parasitica ; Castanea spp. ; genetic variation

分类号：S763

摘要：用RAPD方法,对来自韩国、美国和中国的26个栗疫病菌菌株进行了遗传变异分析。使用筛选的12个随机引物,共扩增了115个0.19～3.1 kb大小的扩增片段,其中多态性片段占61.7%。聚类分析结果,相似系数为0.92时,26个菌株分为两大组,一组由21个菌株组成,包括大部分韩国菌株和美国菌株;另一组包括部分韩国菌株和中国菌株。表明美国菌株和大部分韩国菌株的遗传相似性很高,部分韩国菌株有较大的变异,而中国菌株则表现出了遗传上的远缘关系。
Genetic variation of 26 strains of Cryphonectria parasitica from Korea, USA and China was investigated using random amplified polymorphic DNA （RAPD） of genomic DNA. 115 RAPD bands were amplified by polymerase chain reaction with 12 random primers, and 61.7% of which were polymorphic, the size of amplified RAPD bands ranged from 0.19 to 3.1 kb. The lineage of 26 strains based on cluster analysis of RAPD bands, could be classified as 1 big group and the big group included 17 strains of Korea and 4 strains of USA. From this result, it was showed that most strains of Korea were similar to each other, and also similar to strains of USA, and the genetic relationship with strain of China was very low.