文献类型：期刊文献

英文题名：A non-competitive surface plasmon resonance immunosensor for rapid detection of triazophos residue in environmental and agricultural samples

作者：Guo, Yirong[1] Liu, Rui[1] Liu, Ying[1] Xiang, Dandan[1] Liu, Yihua[1,2] Gui, Wenjun[1] Li, Mingyu[3] Zhu, Guonian[1]

第一作者：Guo, Yirong

通信作者：Liu, Y[1]

机构：[1]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[3]Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China

年份：2018

卷号：613

起止页码：783-791

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20173904200648);Scopus(收录号：2-s2.0-85029719634);WOS:【SCI-EXPANDED(收录号:WOS:000414160500081)】；

基金：This work was financially supported by National Natural Science Foundation of China (No. 31401768), the Special Fund for Agroscientific Research in the Public Interest (No. 201203094-3), and the Special Fund for Forestry Scientific Research in the Public Interest (No. 201304705)

语种：英文

外文关键词：Triazophos; Monoclonal antibody; Direct SPR biosensor; Non-competitive immunoassay

摘要：The wide application of an organophosphate pesticide triazophos raises concern on the environmental pollution and the potential risk to human health. Thus, it is crucial to regularly monitor triazophos residue in the environment and agro-products. Hereinwe described a non-competitive immunoassay for trace detection of triazophos using a direct surface plasmon resonance (SPR) biosensor. Two anti-triazophos monoclonal antibodies (mAbs) were immobilized on the sensor chip and characterized by SPR-based kinetic analysis. The mAb with relatively slowdissociation ratewas used for direct immunosensing of triazophos. The biosensor assay showed a high specificity and a low detection limit of 0.096 ng mL(-1) to triazophos, with the linear detection range of 0.98-8.29 ng mL(-1). Under the optimal condition, the sensor chip could be regenerated for 160 cycles at least. Moreover, the sensitivemethodwas applied to determine triazophos in the spiked environmentalwater and agricultural products, as well as in unknown real-life samples (including Chinese cabbage, cucumber, and apple). Desirable results demonstrated that the newly-developed immunosensor could be used as a rapid, convenient, and reliable tool to regularly monitor triazophos and meet the detection requirement of its maximum residue limits. (C) 2017 Published by Elsevier B.V.