文献类型：期刊文献

中文题名：光质对杉木组培苗增殖及生根生长的影响

英文题名：Effect of Light Quality on Growth and Rooting Growth of Chinese Fir Tissue Culture Plantlets

作者：何平[1] 厉月桥[1] 李迎超[2] 冯慧[3] 谢健杨[4] 孙建军[1]

第一作者：何平

机构：[1]中国林业科学研究院亚热带林业实验中心,江西分宜336600;[2]中国林业科学研究院林业研究所,北京100091;[3]新余学院护理与康复学院,江西新余338004;[4]新余学院经济管理学院,江西新余338004

年份：2019

卷号：25

期号：15

起止页码：25-27

中文期刊名：安徽农学通报

外文期刊名：Anhui Agricultural Science Bulletin

基金：中央级公益性科研院所基本科研业务费专项资金项目“亚林中心杉木优良种质挖掘与利用”（CAFYBB2016MB002）,中央级公益性科研院所基本科研业务费专项资金（CAFYBB2014MB007）项目;中国林科院亚热带林业实验中心省级杉木、马尾松等良种基地省级林业补助专项资金良种繁育项目

语种：中文

中文关键词：光质;杉木;组培苗;增殖;生根

外文关键词：Light quality;Chinese fir;Tissue culture plantlets;Proliferation;Root

分类号：S79

摘要：为了解杉木组培苗增殖及生根过程中的适宜光质,以杉木继代组培苗为试材,研究了不同光质处理对杉木继代组培苗的形态、干重、增殖系数、生根率以及抗氧化酶活性特征等的影响。结果表明:随着组培中红蓝光比例(R/B)的增加,杉木组培苗的增殖系数、丛生芽长度呈逐渐变小的趋势,基茎粗、叶面积、生根率、根长、POD活性以及根系活力值呈先增加后减小的趋势,而细胞内SOD、CAT及MDA的酶活性表现出逐步递增的趋势;R/4B处理时杉木继代组培苗的增殖系数最大为4.93,4R/B处理时丛生芽长达最大值为2.54cm,2R/B处理条件下的生根率与根长达最大值,分别为0.76和1.65cm,均与对照普通光源(W)差异达极显著水平。研究结果可以为杉木组培苗继代培养的光质选择提供理论依据。
In order to clarify the suitable light quality in the process of proliferation and rooting of the tissue culture seedling of Chinese fir,Chinese fir tissue-cultured seedlings as test materials,the morphology,dry weight,multiplication coefficient,rooting rate and antioxidant enzyme activity of Chinese fir subcultured seedlings under different light quality treatments were studied.The results showed that the proliferation coefficient and cluster bud length of Chinese fir tissue-cultured seedlings decreased gradually with the increase of the ratio of red to blue light(R/B)in tissue culture.The basal stem diameter,leaf area,rooting rate,root length,POD activity and root activity in reduc? ing trend after first increase,while SOD,CAT and MDA activity in cells increased gradually.The proliferation coefficient of Chinese fir tissue culture seedling was the highest under R/4B treatment(4.93),the cluster bud length reached the maximum under 4R/B treatment(2.54 cm),and the rooting rate and root length reached the maximum under 2R/B treatment.The large values(0.76 cm,1.65 cm)were significantly different from those of the control common light source(W).The results can provide a basis for the selection of light quality in subculture of Chinese fir seedlings.