文献类型：期刊文献

英文题名：Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

作者：Chang, Ermei[1] Deng, Nan[2] Zhang, Jin[1] Liu, Jianfeng[1] Chen, Lanzhen[3,4] Zhao, Xiulian[1] Abbas, M.[1,5] Jiang, Zeping[1] Shi, Shengqing[1]

第一作者：常二梅

通信作者：Jiang, ZP[1];Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, 1 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China;[2]Hunan Acad Forestry, Inst Ecol, Changsha 410004, Hunan, Peoples R China;[3]Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China;[4]Minist Agr, Risk Assessment Lab Bee Prod Qual & Safety, Beijing 100093, Peoples R China;[5]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China

年份：2018

卷号：66

期号：11

起止页码：3019-3029

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000428356200058)】；

基金：This work was supported by the National Non-Profit Research Institutions of the Chinese Academy of Forestry (RIF2014-11 and CAFYBB2017ZA007), Research Fund of Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration (ZDRIF201707).

语种：英文

外文关键词：Gnetum parvifolium; proteome; seed germination; seed dormancy; iTRAQ

摘要：Gnetum parvifolium is a rich source of materials for traditional medicines, food, and oil, but little is known about the mechanism underlying its seed dormancy and germination. In this study, we analyzed the proteome-level changes in its seeds during germination using isobaric tags for relative and absolute quantitation. In total, 1,040 differentially expressed proteins were identified, and cluster analysis revealed the distinct time points during which signal transduction and oxidation-reduction activity changed. Gene Ontology analysis showed that "carbohydrate metabolic process" and "response to oxidative stress" were the main enriched terms. Proteins associated with starch degradation and antioxidant enzymes were important for dormancy-release, while proteins associated with energy metabolism and protein synthesis were up-regulated during germination. Moreover, protein-interaction networks were mainly associated with heat-shock proteins. Furthermore, in accord with changes in the energy metabolism- and antioxidant-related proteins, indole-3-acetic acid, Peroxidase, and soluble sugar content increased, and the starch content decreased in almost all six stages of dormancy and germination analyzed (S1-S6). The activity of superoxide dismutase, abscisic acid, and malondialdehyde content increased in the dormancy stages (S1-S3) and then decreased in the germination stages (S4-S6). Our results provide new insights into G. parvifolium seed dormancy and germination at the proteome and physiological levels, with implications for improving seed propagation.