文献类型：期刊文献

中文题名：Transcriptional and temporal response of Populus stems to gravi-stimulation

英文题名：Transcriptional and temporal response of Populus stems to gravi-stimulation

作者：Matthew Zinkgraf[1,2] Suzanne Gerttula[1,2] Shutang Zhao[3] Vladimir Filkov[2] Andrew Groover[1,4]

第一作者：Matthew Zinkgraf

机构：[1]USDA Forest Service, Pacific Southwest Research Station, 1731 Research Park Drive, Davis, CA 95618, USA;[2]Department of Computer Science, University of California Davis, One Shields Avenue, Davis, CA 95618, USA;[3]State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;[4]Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, CA 95618, USA

年份：2018

卷号：60

期号：7

起止页码：578-590

中文期刊名：植物学报：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：supported by grant 2015-67013-22891 USDA AFRI to A.G. and V.F.;supported by NSF PGRP Fellowship grant IOS-1402064;supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303

语种：英文

中文关键词：时间;反应;起源;植物响应;基因簇;被子植物;开发;荷尔蒙

外文关键词：Jan Traas;University of Lyon;France

分类号：Q78;O412.1

摘要：Plants modify development in response to external stimuli, to produce new growth that is appropriate for environmental conditions. For example, gravistimulation of leaning branches in angiosperm trees results in modifications of wood development, to produce tension wood that pulls leaning stems upright. Here, we use gravistimulation and tension wood response to dissect the temporal changes in gene expression underlying wood formation in Populus stems. Using time-series analysis of seven time points over a 14-d experiment, we identified8,919 genes that were differentially expressed between tension wood(upper) and opposite wood(lower) sides of leaning stems. Clustering of differentially expressed genes showed four major transcriptional responses, including gene clusters whose transcript levels were associated with Higtwo types of tissue-specific impulse responses that peaked at about 24–48 h, and gene clusters with sustained changes in transcript levels that persisted until the end of the 14-d experiment. Functional enrichment analysis of those clusters suggests they reflect temporal changes in pathways associated with hormone regulation, protein localization, cell wall biosynthesis and epigenetic processes. Time-series analysis of gene expression is an underutilized approach for dissecting complex developmental responses in plants, and can reveal gene clusters and mechanisms influencing development.
Plants modify development in response to external stimuli, to produce new growth that is appropriate for environmental conditions. For example, gravistimulation of leaning branches in angiosperm trees results in modifications of wood development, to produce tension wood that pulls leaning stems upright. Here, we use gravistimulation and tension wood response to dissect the temporal changes in gene expression underlying wood formation in Populus stems. Using time-series analysis of seven time points over a 14-d experiment, we identified 8,919 genes that were differentially expressed between tension wood （upper） and opposite wood （lower） sides of leaning stems. Clustering of differentially expressed genes showed four major transcriptional responses, including gene clusters whose transcript levels were associated withtwo types of tissue-specific impulse responses that peaked at about 24-48h, and gene clusters with sustained changes in transcript levels that persisted until the end of the 14-d experiment. Functional enrichment analysis of those clusters suggests they reflect temporal changes in pathways associated with hormone regulation, protein Iocalization, cell wall biosynthesis and epigenetic pro- cesses. Time-series analysis of gene expression is an underutilized approach for dissecting complex develop- mental responses in plants, and can reveal gene clusters and mechanisms influencing development.