文献类型：期刊文献

英文题名：ESR study in reactive oxygen species free radical production of Pinus kesiya var. langbianensis heartwood treated with laccase

作者：Cao, Y.J.[1,2] Duan, X.F.[1,3] Cao, Y.L.[2] Lü, J.X.[1] Zhu, J.Q.[1] Zhou, G.W.[1] Zhao, B.L.[2]

第一作者：Cao, Y.J.

通信作者：Cao, Y. J.|[a0005f6ab4129b4e6b178]曹永建;

机构：[1] Key Laboratory of Wood Science and Technology of State Forestry Administration, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing, China; [2] Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; [3] Research Institute of Wood Industry, Chinese Academy of Forestry, Wan Shou Shan, Beijing 100091, China

年份：2008

卷号：35

期号：1

起止页码：205-211

外文期刊名：Applied Magnetic Resonance

收录：EI(收录号：20091311984097);Scopus(收录号：2-s2.0-62649142788)

语种：英文

外文关键词：Adhesives - Electron spin resonance spectroscopy - Electrospinning - Fiber bonding - Free radicals - Magnetic moments - Oxygen - pH - Phenolic resins - Urea - Value engineering - Wood products

摘要：Enzymatic oxidation of lignin phenolic hydroxyl groups can enhance the level of autoadhesion between wood fibers or particles depending upon the bonding mechanism of wood-based materials without synthetic adhesives such as urea and phenol formaldehyde. The adhesive effect is caused by an increased number of reactive oxygen groups at the fiber surface. The parameters of laccase-treated wood fibers play vital roles in generating reactive oxygen species (ROS) free radicals. Laccase I (white-rot fungi) and laccase II (Aspergillus sp.) are used to catalyze the oxidation of heartwood powder of Pinus kesiya var. langbianensis in suspension under different pH values, temperatures, treatment times and different laccase concentrations. Electron spin resonance spectroscopy and spin trapping technique were used to detect the ROS free radicals generated in the laccase-treated heartwood powder and it was found that in it the concentration of ROS free radicals was higher than that in the control. Analysis of variance indicates that there was a significant difference between the ROS concentration values of laccase-treated heartwood powder under different pH values, treatment temperatures and times, and laccase dosages for both laccase I and II. Furthermore, the concentration of ROS free radicals generated by laccase I is higher than that generated by laccase II. It was found that the optimum conditions for generation of ROS in laccase-treated heartwood powder by the two kinds of laccase are pH 3.0; treatment temperature, 50 °C; treatment time, 2 h; enzyme concentration, 20 units/g of wood powder. ? 2008 Springer-Verlag.