文献类型：期刊文献

中文题名：烟草和毛白杨次生维管发育相关MYB转录因子分析

英文题名：Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

作者：郭伟[1] 赵树堂[1] 卢孟柱[1]

第一作者：郭伟

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

年份：2016

卷号：29

期号：1

起止页码：25-33

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：“十二·五”863计划课题“林木优质、速生性状调控基因的分离及育种技术研究(2013AA102702)”

语种：中文

中文关键词：烟草;毛白杨;MYB;次生生长

外文关键词：tobacco; Populus tomentosa; MYB; secondary growth

分类号：S792.117;S572

摘要：[目的]烟草具有典型的次生维管发育过程,为了阐明烟草可用于次生维管发育相关基因功能的研究,[方法]通过高通量测序技术获得了烟草维管组织发育的相关MYB转录因子,比对毛白杨基因组数据库,得到烟草和杨树的直系同源基因。利用烟草和毛白杨的基因表达谱,分析了MYB基因表达量变化,并进一步采用荧光定量PCR进行了不同组织的表达分析。[结果]分析得到48对烟草和毛白杨的MYB直系同源基因,可分为23个亚组。15对MYB同源基因在烟草和毛白杨中的表达模式相似。[结论]可以利用烟草所建立的快速基因鉴定系统分析MYB转录因子在维管发育过程中的调控作用。
Tobacco plants have typical secondary vascular development process and can be used to study the function of genes related to secondary vascular development. 48 pairs of MYB orthologous genes obtained from tobacco and poplar were identified by searching in the entire poplar genome sequence with MYB transcription factor obtained from transcriptome of tobacco vascular tissues as probes,and were divided into 23 subgroups. The analysis of gene expression levels obtained using RNA-seq in tobacco and poplar suggested that 15 pairs of MYB orthologous genes shared similar expression patterns both in tobacco and poplar. The results of expression analysis using real time RTPCR in different tissues of tobacco and poplar indicated that the MYB orthologous genes shared similar expression patterns both in tobacco and poplar. It is thus feasible to rapidly analyze the function of MYB transcription factors in vascular development process with tobacco.