文献类型：期刊文献

英文题名：Transcriptome and proteome profiling of adventitious root development in hybrid larch (Larix kaempferi x Larix olgensis)

作者：Han, Hua[1] Sun, Xiaomei[1,2] Xie, Yunhui[2] Feng, Jian[3] Zhang, Shougong[1,2]

第一作者：Han, Hua

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Liaoning Acad Forestry Sci, Forestry Biotechnol & Anal Test Ctr, Shenyang 110032, Liaoning, Peoples R China

年份：2014

卷号：14

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000346930500001)】；

基金：This study was financially supported by the National Science and Technology Support Program (2012BAD01B01) and the National Science Foundation (30972393). The authors thank Dr. Liang Zhang for the service of cDNA library construction and 454 sequencing. The authors are also grateful to Edanz (http://www.liwenbianji.cn/) for their helpful suggestions on improving the manuscript.

语种：英文

外文关键词：Adventitious root development; Hybrid larch; 454 sequencing; 2D-DIGE; MALDI-TOF/TOF-MS; Polyamine synthesis; Stress response

摘要：Background: Hybrids of larch (Larix kaempferi x Larix olgensis) are important afforestation species in northeastern China. They are routinely propagated via rooted stem cuttings. Despite the importance of rooting, little is known about the regulation of adventitious root development in larch hybrids. 454 GS FLX Titanium technology represents a new method for characterizing the transcriptomes of non-model species. This method can be used to identify differentially expressed genes, and then two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analyses can be used to analyze their corresponding proteins. In this study, we analyzed semi-lignified cuttings of two clones of L. kaempferi x L. olgensis with different rooting capacities to study the molecular basis of adventitious root development. Results: We analyzed two clones; clone 25-5, with strong rooting capacity, and clone 23-12, with weak rooting capacity. We constructed four cDNA libraries from 25-5 and 23-12 at two development stages. Sequencing was conducted using the 454 pyrosequencing platform. A total of 957832 raw reads was produced; 95.07% were high-quality reads, and were assembled into 45137 contigs and 61647 singletons. The functions of the unigenes, as indicated by their Gene Ontology annotation, included diverse roles in the molecular functions, biological processes, and cellular component categories. We analyzed 75 protein spots (-fold change >= 2, P <= 0.05) by 2D-DIGE, and identified the differentially expressed proteins using MALDI-TOF/TOF MS. A joint analysis of transcriptome and proteome showed genes related to two pathways, polyamine synthesis and stress response, might play an important role on adventitious root development. Conclusions: These results provide fundamental and important information for research on the molecular mechanism of adventitious root development. We also demonstrated for the first time the combined use of two important technologies as a powerful approach to advance research on non-model, but otherwise important, larch species.