文献类型：期刊文献

中文题名：黄金树B类MADS-box基因表达特征分析

英文题名：Expression Analysis of B-class MADS-box Genes from Catalpa speciosa

作者：景丹龙[1] 夏燕[1] 张守攻[1] 王军辉[1]

第一作者：景丹龙

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,国家林木种质资源平台,北京100091

年份：2016

卷号：51

期号：2

起止页码：210-217

中文期刊名：植物学报

外文期刊名：Chinese Bulletin of Botany

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：十二五国家科技支撑项目(No.2012BAD21B03)

语种：中文

中文关键词：黄金树;花发育;B类基因;基因克隆;基因表达

外文关键词：Catalpa speciosa; flower development; B-class MADS-box genes; gene cloning; the expression of gene;

分类号：Q78

摘要：采用同源克隆技术,从黄金树(Catalpa speciosa)花芽中克隆得到B类MADS-box基因Casp AP3和Casp PI的c DNA序列。序列分析表明,Casp AP3基因c DNA序列的完整开放阅读框(ORF)为696 bp,编码231个氨基酸残基;Casp PI基因c DNA序列的ORF为639 bp,编码212个氨基酸残基。蛋白质序列相似性比对和分子系统发生分析表明,Casp AP3属于AP3/DEF进化支,其C末端包含保守的eu AP3基序和PI-derived基序,而Casp PI聚类于PI/GLO进化支,其C末端包含保守的PI基序。半定量RT-PCR分析结果表明,Casp AP3和Casp PI基因均仅在花瓣和雄蕊中表达。实时荧光定量PCR分析表明,Casp AP3和Casp PI基因在花瓣和雄蕊原基分化期至成熟期均有表达,这2个基因在雄蕊中表达高峰出现的时间均早于花瓣;且花瓣中的Casp AP3和Casp PI基因表达高峰均出现在快速伸长阶段;这与花瓣和雄蕊的形态发育阶段相吻合。
The c DNA sequence of B-class MADS-box genes Casp AP3 and Casp PI involved in flower development were isolated from the flower bud of Catalpa speciosa by homology cloning. The open reading frame（ORF） of Casp AP3 was 696 bp, encoding 231 amino acids, and the ORF of Casp PI was 639 bp, encoding 212 amino acids. Molecular phylogeny analysis and protein sequence alignment suggested that Casp AP3 groups with the AP3/DEF lineages and the C domain of Casp AP3 contained two conserved motifs, eu AP3 and PI-derived motifs. However, Casp PI grouped with the PI/GLO lineages, and the C domain of Casp PI contained a conserved PI motif. Casp AP3 and Casp PI m RNA expression was mainly concentrated in petals and stamens but occurred at the primordium to mature stages of petals and stamens during morphological differentiation of organs. The expression of both factors peaked earlier in stamens than in petals, and the peak expression for both in petals appeared at the stage of rapid elongation. Meanwhile, the time of expression was the same with morphological differentiation of petals and stamens.