文献类型：期刊文献

英文题名：Biohacking agarwood: the impact of fire drills and brine on endophytes and metabolites of Aquilaria sinensis

作者：Zhang, Ningnan[1] Zhou, Xiuren[2] Wang, Shengkun[1] Xu, Daping[1] Hong, Zhou[1] Zhou, Dahao[3] Tang, Deyou[4] Wang, Zhengjian[5] Song, Jie[6] Chen, Jie[1]

第一作者：张宁南

通信作者：Chen, J[1];Song, J[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Peoples R China;[3]Huazhou Yuanlai Agarwood Ltd Co, Huazhou 525100, Peoples R China;[4]South China Univ Technol, Sch Software Engn, Guangzhou 510006, Peoples R China;[5]Huazhou Forestry Bur, Huazhou 525100, Peoples R China;[6]Guangdong Ecoengn Polytech, Sch Tourism & Culture, Guangzhou 510520, Peoples R China

年份：2025

卷号：25

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-105003818068);WOS:【SCI-EXPANDED(收录号:WOS:001478430700002)】；

基金：We thank Dr. Khandaker Md Sharif Uddin Imam for his suggestions and assistance during manuscript writing.

语种：英文

外文关键词：Agarotetrol; Amplicon; GC-MS; Saprotrophs; Symbiotrophs

摘要：Agarwood is a valuable resinous aromatic substance known to have healing properties. Only plants in the Thymelaeace family, specifically the genera Aquilaria and Gyrinops, produce it to defend themselves from insect, bacterial, and fungal attacks. Over-harvesting of natural agarwood has given rise to different artificial agarwood induction techniques. However, the contribution of endophytic microorganisms in this process remains largely unknown. Herein, we employed fire drills and fire drill + brine treatments and investigated their impact on metabolite constituents and endophytes over time. Gas chromatography-mass spectrometry was used to examine the metabolites, and 16 S and ITS amplicon sequencing were applied to check for endophytes. Metabolites from each treatment were related to 16 S and ITS amplicon sequencing results from different times to find out what effect the treatments had and what role endophytes played in making agarwood. Fire drills with 50-80 ml of 0.4 mmol brine treatment resulted in the highest production of essential oil from agarwood, whereas fire drills with 50-80 ml of 4.0 mmol brine treatment produced the highest number of metabolites. Agarwood fragrant compounds such as chromones, sesquiterpenes, and agarotetrol were prominent among the differentially expressed metabolites and were positively associated with the abundance of bacterial endophytes Acidobacteriota, Chlamydiae, Bacteroidota, and Actinobacteria; and the fungal endophytes Rozellomycota, Basidiomycota, Aphelidiomycota, and Mortierellomycota. Saprotrophs and pathogens were prevalent in the treatment groups; however, with time, their proportion declined as Symbiotroph's proportion inclined, indicating successive roles of these fungi in agarwood induction and propagation. Among them, Acidobacteriota, Chlamydiae, Bacteroidota, and Actinobacteria bacterial; and Rozellomycota, Basidiomycota, Aphelidiomycota, and Mortierellomycota fungal endophytes are correlated with enhanced levels of essential agarwood fragrances such as Chromones, Sesquiterpenes, and Agarospirol. Our results show how important bacterial and fungal endophytes are for making agarwood. They also show how these endophytes change when interacting with the host plant after a fire drill and a brine treatment. A moderate brine concentration (0.4 mmol) following a fire drill can thus be employed as a sustainable agarwood production practice.