文献类型：期刊文献

英文题名：Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress

作者：Li, He[1,2,3] Han, Xiaojiao[2,3] Qiu, Wenmin[2,3] Xu, Dong[2,3] Wang, Ying[2,3] Yu, Miao[2,3] Hu, Xianqi[1] Zhuo, Renying[2,3]

第一作者：Li, He

通信作者：Hu, XQ[1];Zhuo, RY[2];Zhuo, RY[3]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Yunnan Agr Univ, Coll Plant Protect, Kunming, Yunnan, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China

年份：2019

卷号：7

期号：4

外文期刊名：PEERJ

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000464917600005)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (No. CAFYBB2016SY008), the National Natural Science Foundation of China (No. 31872168), the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (No. 2016C02056-1) and the Agricultural Projects of Public Scientific and Technology Research Zhejiang Province (No. 2016C32G3030016). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Sedum alfredii Hance; GDSL esterase/lipase proteins (GELPs); Cadmium stress; SaGLIP8

摘要：Background. The herb Sedum alfredii (S. alfredii) Hance is a hyperaccumulator of heavy metals (cadmium (Cd), zinc (Zn) and lead (Pb)); therefore, it could be a candidate plant for efficient phytoremediation. The GDSL esterase/lipase protein (GELP) family plays important roles in plant defense and growth. Although the GELP family members in a variety of plants have been cloned and analyzed, there are limited studies on the family's responses to heavy metal-stress conditions. Methods. Multiple sequence alignments and phylogenetic analyses were performed according to the criteria described. A WGCNA was used to construct co-expression regulatory networks. The roots of S. alfredii seedlings were treated with 100 mu M CdCl2 for qRT-PCR to analyze expression levels in different tissues. SaGLIP8 was transformed into the Cd sensitive mutant strain yeast Delta ycf 1 to investigate its role in resistance and accumulation to Cd. Results. We analyzed GELP family members from genomic data of S. alfredii. A phylogenetic tree divided the 80 identified family members into three Glades. The promoters of the 80 genes contained certain elements related to abiotic stress, such as TC-rich repeats (defense and stress responsiveness), heat shock elements (heat stress) and MYB-binding sites (drought-inducibility). In addition, 66 members had tissue-specific expression patterns and significant responses to Cd stress. In total, 13 hub genes were obtained, based on an existing S. alfredii transcriptome database, that control 459 edge genes, which were classified into five classes of functions in a co-expression subnetwork: cell wall and defense function, lipid and esterase, stress and tolerance, transport and transcription factor activity. Among the hub genes, Sa13F.102 (SaGLIP8), with a high expression level in all tissues, could increase Cd tolerance and accumulation in yeast when overexpressed. Conclusion. Based on genomic data of S. alfredii, we conducted phylogenetic analyses, as well as conserved domain, motif and expression profiling of the GELP family under Cd-stress conditions. SaGLIP8 could increase Cd tolerance and accumulation in yeast. These results indicated the roles of GELPs in plant responses to heavy metal exposure and provides a theoretical basis for further studies of the SaGELP family's functions.