文献类型：期刊文献

英文题名：Screening of Cd tolerant genotypes and isolation of metallothionein genes in alfalfa (Medicago sativa L.)

作者：Wang, Xiaojuan[1] Song, Yu[1,2] Ma, Yanhua[3] Zhuo, Renying[4] Jin, Liang[1]

第一作者：Wang, Xiaojuan

通信作者：Wang, XJ[1]

机构：[1]Lanzhou Univ, Sch Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China;[2]Environm Management Coll China, Qinhuangdao 066004, Peoples R China;[3]Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Peoples R China;[4]Chinese Acad Forest, Res Inst Subtrop Forest, Key Lab Tree Genom, Fuyang 311400, Peoples R China

年份：2011

卷号：159

期号：12

起止页码：3627-3633

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000296397300046)】；

基金：We thank Dr. Hilary Wendt from Royal Holloway University of Landon for helping revised the manuscript. This work was supported by National Basic Research Program of China (2007CB108904) and Fundamental Research Funds for the Central Universities of Lanzhou University (lzujbky-2010-1).

语种：英文

外文关键词：Medicago sativa L.; Cadmium; Metallothionein; Differential display reverse transcription; PCR

摘要：In order to evaluate Cd tolerance in wide-ranging sources of alfalfa (Medicago saliva) and to identify Cd tolerant genotypes which may potentially be useful for restoring Cd-contaminated environments, thirty-six accessions of alfalfa were screened under hydroponic culture. Our results showed that the relative root growth rate varied from 0.48 to 1.0, which indicated that different alfalfa accessions had various responses to Cd stress. The candidate fragments derived from differentially expressed metallothionein (MT) genes were cloned from leaves of two Cd tolerant genotypes, YE and LZ. DNA sequence and the deduced protein sequence showed that MsMT2a and MsMT2b had high similarity to those in leguminous plants. DDRT-PCR analysis showed that MsMT2a expressed in both YE and LZ plant; under control and Cd stress treatment, but MsMT2b only expressed under Cd stress treatment. This suggested that MsMT2a was universally expressed in leaves of alfalfa but expression of MsMT2b was Cadmium (Cd) inducible. (C) 2011 Elsevier Ltd. All rights reserved.