文献类型：期刊文献

英文题名：BEL1-like Homeodomain Protein BLH6a Is a Negative Regulator of CAl5H2 in Sinapyl Alcohol Monolignol Biosynthesis in Poplar

作者：Wang, Qiao[1,2] Dai, Xinren[1] Pang, Hongying[1] Cheng, Yanxia[1] Huang, Xiong[1,2] Li, Hui[1] Yan, Xiaojing[1] Lu, Fachuang[3] Wei, Hairong[4] Sederoff, Ronald R.[5] Li, Quanzi[1,2]

通信作者：Li, QZ[1];Li, QZ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Madison, WI 53705 USA;[3]Wisconsin Energy Inst, Dept Energy, Great Lakes Bioenergy Res Ctr, Madison, WI USA;[4]Michigan Technol Univ, Coll Forest Resources & Environm Sci, Houghton, MI 49931 USA;[5]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC USA

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000671355500001)】；

基金：This work was supported by grants from Fundamental Research Funds of the Chinese Academy of Forestry (CAFYBB2017ZY001 and CAFYBB2016ZX001-1), Natural Science Foundation of China (31670667), and the National Key Research and Development Program of China (2016YFD0600103).

语种：英文

外文关键词：lignin; BEL1-like homeodomain protein; transcription factor; yeast one hybrid

摘要：Lignin is one of the major components of xylem cell walls in tree stems. The lignin in the wood of most flowering plants (dicotyledonous angiosperms) is typically polymerized from three monolignol precursors, coniferyl alcohol, sinapyl alcohol, and p-coumaroyl alcohol, resulting in guaiacyl (G), syringyl (S), and hydroxyphenyl (H) subunits, respectively. In this study, we focus on the transcriptional regulation of a coniferaldehyde 5-hydroxylase (CAld5H2) gene, which encodes a key enzyme for sinapyl alcohol biosynthesis. We carried out a yeast one-hybrid (Y1H) screen to identify candidate upstream transcription factors (TFs) regulating CAld5H2. We obtained 12 upstream TFs as potential regulators of CAld5H2. One of these TF genes, BLH6a, encodes a BEL1-like homeodomain (BLH) protein and negatively regulated the CAld5H2 promoter activity. The direct regulation of CAld5H2 promoter by BLH6a was supported by chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) and dominant repression of BLH6a in transgenic plants. Luciferase complementation imaging analyses showed extensive protein-protein interactions among these 12 TFs. We propose that BLH6a is a negative regulator of CAld5H2, which acts through combinatorial regulation of multiple TFs for sinapyl alcohol (S monolignol) biosynthesis in poplar.