文献类型：期刊文献

英文题名：Facultative symbiosis with a saprotrophic soil fungus promotes potassium uptake in American sweetgum trees

作者：Peng, Long[1,2] Shan, Xiaoliang[1,2] Yang, Yuzhan[2] Wang, Yuchen[1,2] Druzhinina, Irina S.[3] Pan, Xueyu[2] Jin, Wei[2] He, Xinghua[2] Wang, Xinyu[2] Zhang, Xiaoguo[2] Martin, Francis M.[4,5] Yuan, Zhilin[1,2]

第一作者：彭龙

通信作者：Yuan, ZL[1];Martin, FM[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 10091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[3]Nanjing Agr Univ, Coll Resources & Environm Sci, Fungal Genom Grp, Nanjing, Peoples R China;[4]Univ Lorraine, INRA, UMR 1136,INRA Nancy,Lab Excellence ARBRE, Interact Arbres Microorganismes, F-54280 Champenoux, France;[5]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Inst Microbiol, Beijing, Peoples R China

年份：2021

卷号：44

期号：8

起止页码：2793-2809

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-85106707508);WOS:【SCI-EXPANDED(收录号:WOS:000655632300001)】；

基金：Fundamental Research Funds for the Central Non-profit Research of Chinese Academy of Forestry, Grant/Award Number: CAFYBB2020QB002; Martin's lab is funded by the Laboratory of Excellence ARBRE, Grant/Award Number: ANR-11-LABX-0002_ARBRE; National Natural Science Foundation of China, Grant/Award Numbers: 31722014, 31901290

语种：英文

外文关键词：endophytes; mycorrhizal fungi; RNA-seq; root-fungus symbiosis; soil saprotrophs; soluble sugars

摘要：Several species of soil free-living saprotrophs can sometimes establish biotrophic symbiosis with plants, but the basic biology of this association remains largely unknown. Here, we investigate the symbiotic interaction between a common soil saprotroph, Clitopilus hobsonii (Agaricomycetes), and the American sweetgum (Liquidambar styraciflua). The colonized root cortical cells were found to contain numerous microsclerotia-like structures. Fungal colonization led to increased plant growth and facilitated potassium uptake, particularly under potassium limitation (0.05 mM K+). The expression of plant genes related to potassium uptake was not altered by the symbiosis, but colonized roots contained the transcripts of three fungal genes with homology to K+ transporters (ACU and HAK) and channel (SKC). Heterologously expressed ChACU and ChSKC restored the growth of a yeast K+-uptake-defective mutant. Upregulation of ChACU transcript under low K+ conditions (0 and 0.05 mM K+) compared to control (5 mM K+) was demonstrated in planta and in vitro. Colonized plants displayed a larger accumulation of soluble sugars under 0.05 mM K+ than non-colonized plants. The present study suggests reciprocal benefits of this novel tree-fungus symbiosis under potassium limitation mainly through an exchange of additional carbon and potassium between both partners.