文献类型：期刊文献

英文题名：Sequence Analysis and Functional Verification of the Effects of Three Key Structural Genes, PdTHC2'GT, PdCHS and PdCHI, on the Isosalipurposide Synthesis Pathway in Paeonia delavayi var. lutea

作者：Zou, Hongzhu[1] Han, Lulu[1] Yuan, Meng[1] Zhang, Mengjie[1] Zhou, Lin[1] Wang, Yan[1]

第一作者：Zou, Hongzhu

通信作者：Zhou, L[1];Wang, Y[1]|[a00054d9438f8e4632f3b]周琳;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2022

卷号：23

期号：10

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000801832600001)】；

基金：This research was funded by grants from the National Key Research and Development Program (Grant No. 2018YFD1000405), and the National Natural Science Foundation of China (Grant No. 31972456).

语种：英文

外文关键词：Paeonia delavayi var; lutea; isosalipurposide; PdTHC2'GT; PdCHS; PdCHI; functional verification

摘要：Isosalipurposide (ISP) is the most important yellow pigment in tree peony. In ISP biosynthesis, CHS catalyzes 1-molecule coumaroyl-CoA and 3-molecule malonyl-CoA to form 2 ',4 ',6 ',4-tetrahyroxychalcone (THC), and THC generates a stable ISP in the vacuole under the action of chalcone2 '-glucosyltransferases (THC2 ' GT). In tree peony, the details of the THC2'GT gene have not yet been reported. In this study, the candidate THC2'GT gene (PdTHC2'GT) in Paeonia delavayi var. lutea was screened. At the same time, we selected the upstream CHS gene (PdCHS) and the competitive CHI gene (PdCHI) to study the biosynthesis pathway of ISP. We successfully cloned three genes and sequenced them; subcellular localization showed that the three genes were located in the nucleus and cytoplasm. The overexpression of PdTHC2'GT in tobacco caused the accumulation of ISP in tobacco petals, which indicated that PdTHC2'GT was the key structural gene in the synthesis of ISP. After the overexpression of PdCHS and PdCHI in tobacco, the accumulation of anthocyanins in tobacco petals increased to different degrees, showing the role of PdCHS and PdCHI in anthocyanin accumulation. The analysis of NtCHS and NtCHI of transgenic tobacco lines by qRT-PCR showed that the THC2'GT gene could increase the expression of CHS. THC2'GT and CHI were found to be competitive; hence, the overexpression of THC2'GT could lead to a decrease in CHI expression. The CHS gene and CHI gene could increase the expression of each other. In conclusion, we verified the key structural gene PdTHC2'GT and studied the operation of the genes in its upstream and competitive pathway, providing a new perspective for the biosynthesis of ISP and new candidate genes for the directional breeding of tree peony.