文献类型：期刊文献

中文题名：尾细桉YL02叶片诱导的植株再生体系的建立

英文题名：Establishment of Plant Regeneration System of Eucalyptus urophylla ×E. tereticornis Clone YL02 Leaves Induction

作者：范春节[1] 曾炳山[1] 裘珍飞[1] 刘杰[1] 郭光生[1]

第一作者：范春节

机构：[1]中国林业科学研究院热带林业研究所

年份：2016

卷号：0

期号：10

起止页码：2785-2790

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家科技计划项目(2013AA102705);中央级公益性科研院所基本科研业务费专项资金项目(RITFYWZX201304)共同资助

语种：中文

中文关键词：尾细桉;叶片;愈伤组织诱导;不定芽再生

外文关键词：Eucalyptus urophylla×E. tereticornis;;Leaf;;Callus induction;;Adventitious bud regeneration

分类号：S792.39

摘要：以尾细桉无菌生根苗叶片为外植体,通过调整培养基中TDZ、NAA和磷酸二氢钾浓度和外植体状态和部位进行诱导植株再生研究,以期建立起尾细桉叶片诱导的再生体系。结果表明:TDZ、NAA和磷酸二氢钾浓度对叶片诱导植株再生呈显著性影响,同时外植体状态和部位对叶片诱导植株再生也起到明显的作用。通过试验,最终得到在0.025 mg/L TDZ+0.05 mg/L NAA加入170 mg/L的磷酸二氢钾的培养基中,同时以在生根培养基上生长25 d生根苗顶端起向下1-4片叶为外植体获得了最高72.7%的植株再生效率,诱导芽为丛芽,芽再生部位为叶柄和叶脉中伤口部位,外植体平均芽数达到14.14个,初步建立了尾细桉叶片诱导的遗传转化体系,本研究为下一步尾细桉高效稳定遗传转化体系研究提供研究基础。
The effects of plant growth regulators thidiazuron(N-phenyl-N'-1,2,3-thidiazol-5-yl urea; TDZ) and1-naphthaleneacetic acid(NAA) and Potassium Phosphate Monobasic on callus induction and plant regeneration were studied by using Eucalyptus urophylla ×E.tereticornis clone YL02 leaves as explants at different position of plant and treatment time at root induction medium. Results showed that different TDZ, NAA and Potassium Phosphate Monobasic concentrations and explants condition had significant influences on callus induction and adventitious bud regeneration. The highest induction rate of adventitious buds was 72.7%, which induced directly from upper leaves(first to forth from apex) of plants grown 25 days under root induction medium. The best medium was m MS supplemented with 0.025 mg/L TDZ, 0.05 mg/L NAA and 170 mg/L Potassium Phosphate Monobasic.Meanwhile, the induced adventitious buds were multiple buds and average buds reached 14.14 per explant. This study could offer research foundation for the establishment of stable and efficient E. urophylla ×E.tereticornis genetic transformation system.