文献类型：期刊文献

英文题名：Molecular cloning and functional analysis of the Populus deltoides remorin gene PdREM

作者：Li, Shaofeng[1] Su, Xiaohua[2] Zhang, Bingyu[2] Huang, Qinjun[2] Hu, Zanmin[3] Lu, Mengzhu[2]

第一作者：李少锋

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, Forestry Expt Ctr North China, State Key Lab Tree Genet & Breeding, Beijing 100023, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

年份：2013

卷号：33

期号：10

起止页码：1111-1121

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000327460000010)】；

基金：This work was supported by grants from the Major State Basic Research Development Program of China (973 program) (2012CB114506) and the "Twelfth Five-Year" National Science and Technology Support Program (2012BADO1BO3).

语种：英文

外文关键词：molecular mechanisms; PdREM; Populus deltoides; wood formation

摘要：Remorins play vital roles in signal transduction, energy transformation, ion flow and transport in plants. Upregulation of remorins correlates with dehiscence and cell maturation; however, no studies have been performed to elucidate the function of remorins in tree species. In this study, a Populus deltoides (Marsh.) plasma membrane-binding protein remorin gene (PdREM) was cloned and characterized by investigating its expression pattern and creating transgenic hybrid poplar (P. davidiana Dode x P. bolleana Lauche) lines expressing sense or antisense PdREM. PdREM was specifically expressed in leaf buds, and immature and mature phloem in P. deltoides. Downregulation of PdREM increased plant height, stem diameter, number of leaves, size of the xylem and phloem zones and induced expression of cell wall biosynthesis- and microfibril angle (MFA)-related genes. Overexpression of PdREM retarded vegetative growth. PdREM may negatively regulate vascular growth by inhibiting secondary cell wall expansion in poplar. In addition, antisense PdREM transgenic poplar had a lower MFA, suggesting that PdREM might contribute to sheet strength and wood properties in poplar. This study sheds light on the molecular mechanism of PdREM in P. deltoides growth and development, and lays the foundation for future functional genomics research into wood formation and the genetic engineering of forest trees with improved wood quality traits.