文献类型：期刊文献

英文题名：A genome-wide survey of microRNA truncation and 3' nucleotide addition events in larch (Larix leptolepis)

作者：Zhang, Junhong[1,2] Zhang, Shougong[1] Li, Shuigen[1] Han, Suying[3] Wu, Tao[1] Li, Xinmin[1,4] Qi, Liwang[1]

第一作者：Zhang, Junhong

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Cell Biol Lab, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Zhejiang Agr & Forestry Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Key Lab, Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[4]Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

年份：2013

卷号：237

期号：4

起止页码：1047-1056

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000316759800012)】；

基金：This work was supported by the National Basic Research Program of China (Grant Nos. 2009CB119106), the National Natural Science Foundation of China (Grant Nos. 30830086), and the National High Technology Research and Development Program of China (Grant Nos. 2011AA100203).

语种：英文

外文关键词：MicroRNA; MicroRNA truncation; IsomiR; Cytidine addition; Larix leptolepis

摘要：MicroRNAs (miRNAs) play essential roles in numerous developmental and metabolic processes in animals and plants. Although the framework of miRNA biogenesis and function is established, the mechanism of miRNA degradation or modification remains to be investigated in plants. Mature miRNAs may be truncated or added nucleotides to generate variants. A detailed analysis of small RNA deep sequencing data sets resulted in the cloning of a large number of variants derived from larch miRNAs. Many 5'- and/or 3'-end truncated versions of miRNAs suggested that larch miRNAs might be degraded through either 5'aEuro"3' or 3'aEuro"5'. The relative abundance of variants truncated from 3'-end was higher than that of 5'-end for most miRNAs. The addition of adenine, uridine, and cytidine to the 3'-end of miRNAs was globally present, and the subtle variability in isomiR abundance might be regulated and biologically meaningful. It is the first report for cytidine addition in plant, and our examination of published small RNA deep sequencing data sets of Arabidopsis, rice, and moss suggests that cytidine addition to miRNA 3'-end exists broadly in plants. In addition, the nucleotide addition might be associated with 3'aEuro"5' miRNA degradation. Our results provide valuable information for a genome-wide survey of miRNA truncation and modification in larch or plants.