文献类型：期刊文献

中文题名：火炬松、湿地松、晚松组培繁殖的研究

英文题名：Studies on the Tissue Cultue of Pinus taeda, P.elliottii and P.serotina

作者：阙国宁[1] 房建军[1] 葛万川[1] 张守英[1] 毛秋娟[1]

第一作者：阙国宁

机构：[1]中国林业科学研究院亚热带林业研究所

年份：1997

卷号：10

期号：3

起止页码：227-232

中文期刊名：林业科学研究

外文期刊名：ROREST RESEARCH

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金："八五"攻关项目

语种：中文

中文关键词：火炬松;湿地松;晚松;繁殖;组织培养

外文关键词：Pinus taeda L. P.elliottii E. P.serotina M. tissue culture propagation

分类号：S791.240.4

摘要：本试验以火炬松、湿地松、晚松成熟种子的胚为材料，在附加有细胞分裂素（ＢＡＰ）的ＧＤ改良基本培养基（总盐分含量为０．２１％；总含氮量为１８６ｍｇ／Ｌ）经３～４周培养，在胚或子叶及下胚轴上出现膨大肉眼可见的暗绿色愈伤组织及小芽，其分化率达７５％～８５％。培养基中的细胞分裂素（ＢＡＰ）浓度对三种松种不定芽的分化具有很大影响，一般以３～５ｍｇ／Ｌ为好，在继代培养基中，活性炭对于嫩梢的生长具有良好的效果。当嫩梢长至１．５ｃｍ时，可供生根。在含有０．５ｍｇ／ＬＮＡＡ的生根培养基中经１５～２０ｄ培养，即长出白色愈伤组织，然后转至无激素培养基中培养１５～２０ｄ后生根。生根的小植株移栽入土壤，其成活率可达８０％～９５％。
The embryos,of the mature seeds of P.taeda L., P.elliottii E., P.serotina M.,were used as experimental material. The explants were placed on modified GD medium(total salt 0.21%,total nitrogen 186 mg/L) suplemented with BAP for 3～4 weeks to induce swollen dark green callus,then the buds were found on embryos,cotyledon and hypocotyl.The rate of differentiation reached 75%～85%,and the concentration of BAP played an important part in the differentiation of adventiation buds of three pines,normally 3～5 mg/L is suitable.They were subcultured to medium containing activated charcoal,which is benefical to the growth of shoots.When the shoots grown to the length of 1.5 cm,they were planted on the rooting medium with 0.5 mg/L NAA for 15～20 days,and the white callus were visible.At this point they were transferred to the hormone free medium for 15～20 days to root,when the plantlets were planted in soil,85%～90% of them have survived.