文献类型：期刊文献

英文题名：Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

作者：Wang, Zhi[1] Zhu, Tianqing[1] Ma, Wenjun[1] Fan, Erqin[1,3] Lu, Nan[1] Ouyang, Fangqun[1] Wang, Nan[1] Yang, Guijuan[1] Kong, Lisheng[2] Qu, Guanzheng[3] Zhang, Shougong[1] Wang, Junhui[1]

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Univ Victoria, Dept Biol Ctr Forest Biol, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Peoples R China

年份：2020

卷号：20

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000546960900001)】；

基金：This work was supported by Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002); Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZA001-8). The funders had no role in the study design, data analysis and interpretation, and manuscript writing, but just provided the financial.

语种：英文

外文关键词：Catalpa bungei; Flowering; SPL; HMGA; Architectural transcriptional factor

摘要：Background: "Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results: SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. Conclusions: Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.