文献类型：期刊文献

中文题名：美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较

英文题名：Comparison of Nucleic Acid Contents Among Non-diapause Pupae,Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio memnon

作者：易传辉[1,2] 陈晓鸣[1] 史军义[1] 周成理[1]

第一作者：易传辉

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室;[2]云南林业职业技术学院

年份：2009

卷号：37

期号：11

起止页码：5018-5019

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

收录：北大核心:【北大核心2008】；

基金：国家林业局引进国际先进林业科学技术计划项目(2005-4-59和2008-4-68)

语种：中文

中文关键词：美凤蝶;核酸含量;滞育;蛹;成虫

外文关键词：Papilio memnon;Nucleic acid content;Diapause;Pupae;Dault

分类号：Q966

摘要：[目的]为深入了解美凤蝶滞育机理提供依据。[方法]利用比色法,测定非滞育蛹为、滞育蛹和滞育蛹羽化成虫不同发育阶段RNA和DNA含量。[结果]非滞育蛹RNA含量为4.614 0～7.946 3μg/mg,滞育蛹为4.326 0～5.885 3μg/mg,羽化成虫初期为20.7793μg/mg;非滞育蛹DNA含量为0.448 7～0.535 0μg/mg,滞育蛹为0.452 0～0.828 3μg/mg,羽化成虫初期为1.727 0μg/mg。[结论]核酸含量与变化与发育阶段有关。
[Objective] The study aimed to provide basis for knowing the diapause mechanism of Papilio memnon L.deeply.[Method] Using colorimetry,the nucleic acid content of non-diapause pupae,diapause pupae and eclosion-adult from diapause pupae were examined respectively.[Result] The result showed that the RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg,diapause pupae was 4.326 0-5.885 3 μg/mg,and eclosion-adult from diapause pupae was 20.779 3 μg/mg.The DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg,diapause pupae were 0.452 0-0.828 3 μg/mg,and eclosion-adult from diapause pupae was 1.727 0 μg/mg.[Conclusion]The contents and transformation of nucleic acid were related to the different stage of development.