文献类型：期刊文献

中文题名：普通油茶无性系花粉离体萌发特性

英文题名：Characteristics of Pollen Germination in Vitro of Camellia oleifera

作者：李春林[1,2] 杨水平[1] 姚小华[2] 任华东[2] 王开良[2] 林萍[2]

第一作者：李春林

机构：[1]西南大学资源环境学院;[2]中国林业科学研究院亚热带林业研究所

年份：2011

卷号：24

期号：2

起止页码：212-217

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金："十一五"国家科技支撑计划"油茶高产优质新种质创制"(2009BADB1B01);中国林科院基本科研业务费专项资金项目"高油高抗油茶杂交新种质创制"(CAFYBB2008005)

语种：中文

中文关键词：普通油茶;花粉离体;萌发特性

外文关键词：Camellia oleifera; pollen in vitro; germination characteristic

分类号：S794.4

摘要：以6个普通油茶无性系花粉为试验材料,采用正交试验研究了不同温度、硼元素用量、蔗糖浓度、琼脂用量对花粉萌发的影响,以揭示普通油茶花粉在离体条件下的萌发特性。结果表明:4个影响花粉萌发率的试验因素中,温度是极显著影响因素,以25℃最优;蔗糖浓度也能显著影响多数无性系,以10%最优;硼元素用量和琼脂用量仅对个别无性系有显著影响,分别以100 mg·kg-1和0.5%最优。结合多重比较,处理T9为试验优选组合;花粉管的生长从开始到停止略呈"慢—快—慢"的抛物线趋势;稍高的钙离子浓度显著抑制花粉萌发,低浓度锌、钼离子对花粉萌发略有促进,高浓度则效应为负。
To study the characteristics of pollen germination in vitro of six Camellia oleifera species,the orthogonal test using six C.oleifera species as the materials was aimed to examine the effects of the 4 factors consisting of temperature,boron consumption,sucrose,and agar usage on pollen germination.The results indicated that temperature was the most significant factor among the 4 factors and the optimum temperature was 25 ℃,sucrose markedly affected the pollen germination of most species and the optimum sucrose was 10%,and boron consumption and agar usage markedly impacted on individual clones and the optimum were 100 mg·kg^-1 and 0.5%.Combining multiple comparision,the optimum extractive condition is T9;the growth of pollen tube followed the ＂slow-fast-slow＂ trent.When the concentration of Ca^2＋ was high,the pollen germination was markedly inhibited.If the concentrations of Zn^2＋ and Mo^6＋ were low,the pollen tube growth would be enhanced.But when the concentrations of Zn^2＋ and Mo^6＋ were high,the pollen germination of pollen tube was negative effected.