文献类型：期刊文献

中文题名：不同生长势毛棉杜鹃根部内生真菌多样性差异

英文题名：Differences in Root Endophytic Fungal Diversity of Rhododendron moulmainense with Different Growth Potentials

作者：庄鹏[1,2] 彭金根[2] 刘思佳[2] 白宇清[3] 张露文[1,2] 李荣生[1] 杨锦昌[1] 谢利娟[2] 蔡洪月[2]

第一作者：庄鹏

机构：[1]中国林业科学研究院热带林业研究所,广州510520;[2]深圳职业技术大学建筑工程学院,深圳518055;[3]深圳市梧桐山风景区管理处,深圳518004

年份：2025

卷号：61

期号：5

起止页码：61-73

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：;EI(收录号：20252218516434);北大核心:【北大核心2023】；

基金：深圳职业技术大学科研启动项目(6022312013K);毛棉杜鹃在深圳的推广应用研究(1027-6019260135K1)。

语种：中文

中文关键词：扩增子ITS测序;毛棉杜鹃;内生真菌;组织分离培养;生长势;根;真菌群落

外文关键词：ITS sequencing of amplicons;Rhododendron moulmainense;endophytic fungi;tissue isolation and culture;growth potential;root;fungal community

分类号：S685.21;S718.81

摘要：【目的】对生长于深圳市梧桐山的不同生长势的毛棉杜鹃根系内生真菌类群和多样性进行分析,筛选出具有代表性和对毛棉杜鹃生长有潜在积极作用的内生真菌,为毛棉杜鹃内生真菌资源开发利用提供参考。【方法】针对不同生长势的毛棉杜鹃根系,通过扩增子ITS高通量测序和组织分离培养法,分析根系内生真菌类群和多样性。【结果】1)通过毛棉杜鹃根系真菌的扩增子ITS高通量测序分析,鉴定获得生长势好的样本183942条序列,注释出11门231种真菌,778个操作分类单元(OTU);鉴定获得生长势差的样本190918条序列,注释出10门202种真菌,1009个OTU,担子菌门和子囊菌门是2种生长势中的主导菌门。2)α多样性分析显示:生长势好的毛棉杜鹃根系真菌群落的多样性高于生长势差的毛棉杜鹃;PcoA多样性分析表明,相同生长势的群落间相似性高,而不同生长势的群落间存在差异。3)功能预测分析显示:在生长势好的毛棉杜鹃根系真菌群落中,共生营养型真菌占比近60%;而在生长势差的群落中,腐生和共生营养型真菌各占约40%。4)基于组织分离培养法共鉴定出172种内生真菌,子囊菌门是2种生长势毛棉杜鹃根系真菌的主导菌门;生长势好、差的植株根系分别鉴定出98、109种真菌;从生长势好的毛棉杜鹃中分离出12株代表性真菌,生长势差的分离出6株代表性真菌;典型的杜鹃花类菌根真菌有树粉孢属类真菌和欧石楠无柄盘真菌,仅在生长势好的毛棉杜鹃根系中分离出。【结论】扩增子ITS高通量测序技术和组织分离技术揭示了不同生长势毛棉杜鹃根系内生真菌群落的多样性存在差异,相较于生长势差的植株,生长势好的毛棉杜鹃根系中具有物种丰度更高和群落结构分布更加均衡的内生真菌群落,其中优势真菌群落树粉孢属、无柄盘菌属的种类和数量较多。本研究证实毛棉杜鹃根系微生物生态与植物生长势之间的潜在联系,为进一步探索植物-微生物相互作用提供了新视角。
【Objective】This study aimed to analyze the endophytic fungal communities and diversity in the roots of Rhododendron moulmainense with varying growth potential in Wutong Mountain,Shenzhen,China.The goal was to identify representative endophytic fungi with potential benefits for plant growth and lay a foundation for utilizing these fungal resources.【Method】Root samples from R.moulmainense with different growth potential were analyzed using amplicon ITS highthroughput sequencing and tissue isolation cultivation to characterize fungal taxa and diversity.【Result】1)Amplicon ITS high throughput sequencing of root associated fungi in R.moulmainense revealed distinct community profiles between good and poor growth potential samples.For good growth potential roots,183942 sequences were annotated into 11 phyla,231 fungal species,and 778 operational taxonomic units(OTUs).In contrast,poor growth potential roots yielded 190918 sequences,annotated into 10 phyla,202 fungal species,and 1009 OTUs.Despite differences in taxonomic richness,Basidiomycota and Ascomycota emerged as the dominant phyla across both growth potential groups.2)Alpha diversity analysis revealed that the fungal communities in the roots of R.moulmainense with good growth potential exhibited greater diversity compared to those with poor growth potential.Principal Coordinate Analysis(PCoA)further demonstrated that communities sharing the same growth potential clustered closely,indicating high similarity,whereas communities from differing growth potential levels showed divergence.3)Functional prediction analysis demonstrated that in the fungal communities associated with the roots of good growth potential R.moulmainense,symbiotrophic fungi accounted for nearly 60%of the total community.In contrast,fungal communities from poor growth potential roots exhibited a balanced distribution,with both saprotrophic fungi and symbiotrophic fungi each comprising approximately 40%.4)Through tissue isolation and cultivation methods,a total of 172 endophytic fungal species were identified in the roots of R.moulmainense.Ascomycota dominated the fungal communities in both good and poor growth potential plants.Specifically,98 fungal species were identified in good growth potential roots,while 109 species were found in poor growth potential roots.Among these,12 representative fungal strains were isolated from good growth potential plants,compared to 6 strains from poor growth potential plants.Notably,typical Ericoid mycorrhizal fungi,including Oidiodendron and Pezicula ericae,were exclusively isolated from good growth potential roots.【Conclusion】Amplicon ITS high-throughput sequencing and tissue isolation techniques revealed significant differences in the diversity of endophytic fungal communities within the roots of R.moulmainense exhibiting different growth potential.Compared to plants with poor growth potential,those with robust growth demonstrated a higher abundance of endophytic fungi,particularly dominated by taxa such as Oidiodendron and P.ericae,which were both more diverse and numerically prevalent.This discovery underscores a potential link between the root-associated mycobiome ecology of R.moulmainense and its growth performance,providing novel perspectives for further exploration of plant-microbe interactions and their role in enhancing plant resilience and vitality.