文献类型：期刊文献

中文题名：银腺杨PagWOX11/12a基因对茎生长发育的影响

英文题名：Effects of PagWOX11/12a Gene on Stem Growth and Development of Populus alba×P. glandulosa

作者：文爽爽[1] 王留强[1,2] 卢孟柱[1,2,3]

第一作者：文爽爽

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,北京100091;[2]南京林业大学,南方现代林业协同创新中心,江苏南京210037;[3]浙江农林大学,省部共建亚热带森林培育国家重点实验室,浙江杭州311300

年份：2023

卷号：36

期号：1

起止页码：39-46

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(31971620)。

语种：中文

中文关键词：PagWOX11/12a;银腺杨;节间伸长;株高;功能分析

外文关键词：PagWOX11/12a;Populus alba×P.glandulosa;internode elongation;plant height;functional analysis

分类号：S718.46

摘要：[目的]分析PagWOX11/12a基因对杨树生长发育的影响,为进一步研究该基因在木本植物中的调控机制奠定基础。[方法]利用生物信息学方法及相关软件进行系统发育进化树构建、序列比对及生化特征分析。利用qRT-PCR分析其组织特异性表达模式。利用转基因植株35S::PagWOX11/12a-SRDX(DR),分析显性抑制PagWOX11/12a后杨树的表型。[结果] PagWOX11/12a基因可编码含255个氨基酸的蛋白质,该基因在84K的不同组织中均有表达。对DR转基因植株的表型分析表明,与非转基因植株相比,显性抑制该基因可导致茎中韧皮部细胞、髓心细胞及木质部纤维细胞长度变小,节间伸长受到抑制,株高明显降低。[结论]PagWOX11/12a基因通过影响节间伸长参与调控了杨树的高生长,该研究为进一步揭示PagWOX11/12a基因参与杨树生长发育的调控机制提供了参考。
[Objective]To analyze the effects of PagWOX11/12a gene on the growth and development of poplar for further research on the regulation mechanism of this gene in woody plants. [Method]Bioinformatics methods and software were used to construct phylogenetic evolutionary tree, sequence alignment and biochemical characterization analysis. Tissue-specific expression patterns were analyzed by qRTPCR. The phenotype of poplar after specifically suppressed the expression of PagWOX11/12a was analyzed by using transgenic plant 35S::PagWOX11/12a-SRDX(DR). [Result]PagWOX11/12a gene could encode a protein with 255 amino acids, which was expressed in different tissues of 84K. The phenotypic analysis of DR transgenic plants showed that inhibiting the expression of this gene could reduce the length of phloem cells, pith cells and xylem fiber cells, inhibit internode elongation, and significantly reduced plant height compared with non-transgenic 84K. [Conclusion]PagWOX11/12a gene participates in regulating the height growth of poplar by affecting the elongation of internodes. This study provides a reference for further revealing the regulatory mechanism of PagWOX11/12a gene involved in the growth and development of poplar.