文献类型：期刊文献

中文题名：嘧菌酯高效液相色谱分析方法及防腐材抗流失性能研究

英文题名：Analytical method of azoxystrobin by HPLC and leaching performance of treated wood

作者：张景朋[1] 蒋明亮[1] 张斌[1]

机构：[1]中国林业科学研究院木材工业研究所,北京100091

年份：2025

卷号：42

期号：1

起止页码：185-192

中文期刊名：浙江农林大学学报

外文期刊名：Journal of Zhejiang A&F University

收录：;北大核心:【北大核心2023】；

基金：中国林业科学研究院院基金重点资助项目(CAFYBB2020ZA003);国家自然科学基金资助项目(32301525)。

语种：中文

中文关键词：木材保护;嘧菌酯;高效液相色谱(HPLC);防腐材;抗流失性

外文关键词：wood protection;azoxystrobin;high performance liquid chromatography(HPLC);treated wood;leaching resistance

分类号：S782.3

摘要：【目的】探究嘧菌酯(azoxystrobin)的高效液相色谱(HPLC)分析方法及抗流失性能,旨在确定嘧菌酯处理材的抗流失性能,为其户外使用提供研究基础。【方法】采用紫外检测器及C_(18)反相色谱柱,通过优化检测波长、流动相组成及配比、柱温及流动相流速等色谱条件,确定分析嘧菌酯的色谱条件,采用外标法对嘧菌酯质量浓度进行检测,并进行标准曲线相关系数、精密度、准确度等分析;参照GB/T 29905—2013《木材防腐剂流失率试验方法》开展流失试验,并利用建立的分析方法测定各处理材流失液中嘧菌酯的质量浓度,以确定防腐材的抗流失性能。【结果】优化得到嘧菌酯分析的色谱条件:V(甲醇):V(水)=80:20为流动相,流速为0.8 mL·min^(-1),检测波长为220 nm。嘧菌酯标准溶液在质量浓度为1~80 mg·L^(-1)内,标准曲线相关系数为0.999 6,线性相关性强;2个分析样品的变异系数为0.45%和0.59%,样品的回收率为98.2%~100.4%。抗流失试验结果表明:处理材中嘧菌酯的抗流失性较好,马尾松Pinus massoniana、杉木Cunninghamia lanceolata和107杨Populus×euramericana处理材的流失率分别为8.4%~18.8%、8.2%~21.0%和8.8%~19.0%。3种处理材之间的嘧菌酯流失率差异不大,均表现为低质量分数制剂处理材的流失率大于高质量分数制剂处理材的规律;乙醇溶解嘧菌酯处理材的流失率稍高于2种水基化制剂处理材的流失率。【结论】采用HPLC测定嘧菌酯的质量浓度,标准曲线线性相关性强,精密度和准确度高,能准确分析嘧菌酯的质量浓度;嘧菌酯的2种水基化制剂的抗流失性能较好,可用于户外木材及其制品。
[Objective]This study,with an exploration of the high performance liquid chromatography(HPLC)analysis method and leaching resistance of the azoxystrobin formulation,is aimed to provide a research basis for its outdoor utilization.[Method]On the one hand,UV detector and C18 reversed-phase chromatographic column were used for detection before an investigation was conducted of the chromatographic conditions,such as detection wavelength,flow phase composition and ratio,column temperature and flow phase flow rate with further discussion so as to optimize the chromatographic conditions suitable for the analysis of azoxystrobin.On the other hand,the mass concentration of azoxystrobin was detected by external standard method,and the standard curve correlation coefficients,precision and accuracy were evaluated after which the leaching procedure was adopted according to the Chinese standard GB/T 29905-2013 Laboratory Method of Determining the Leachability of Wood Preservatives,and leaching rate of azoxystrobin in treated wood were tested by the established analytical method.[Result]The optimized chromatographic conditions for analysis of azoxystrobin were as follows:flow phase methanol-water ratio of 80∶20,flow rate of 0.8 mL·min^(-1),and UV detection wavelength of 220 nm.There was a good linear relationship between azoxystrobin concentration and chromatographic peak area in the range of 1-80 mg·L^(-1) for azoxystrobin standard solution,with a determination coefficient of 0.9996.The coefficient variation of samples were 0.45%and 0.59%,and the recovery rates of the samples were 98.2%-100.4%.The leaching rate of azoxystrobin from Pinus massoniana,Cunninghamia lanceolata and Populus×euramericana were 8.4%-18.8%,8.2%-21.0%and 8.8%-19.0%,respectively,with little difference in leaching rates between the three treated woods.The leaching rate of azoxystrobin in the treated Pinus massoniana,C.lanceolata and Populus×euramericana were all below 21%and the leaching resistance were excellent.All of the three tree species exhibit a pattern where the leaching rate of low concentration treated woods is greater than that of high concentration treated woods whereas the leaching rate of the treated wood with ethanol-dissolved azoxystrobin was slightly higher than that of the water-based formulations.[Conclusion]The HPLC method for determining azoxystrobin mass concentration showed good linear correlation of the standard curve,high precision,and recovery rate,which can be used for analysis of azoxystrobin mass concentration.The leaching resistance of the two water-based formulations of azoxystrobin was good and can be used for outdoor treatment of wood and its products.