文献类型：期刊文献

英文题名：Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation

作者：Jiang, Peng-Fei[1] Lin, Xiao-Yang[2] Bian, Xiu-Yan[1] Zeng, Qing-Yin[1] Liu, Yan-Jing[1]

第一作者：Jiang, Peng-Fei

通信作者：Liu, YJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China

年份：2022

卷号：172

起止页码：24-32

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000783431500003)】；

基金：Acknowledgements This work was supported by the National Natural Science Foundation of China (31822011) , the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2018ZX001 and CAFYBB2019ZY002) , and the fund of the State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry (TGB2019001) . We thank Yuan Cao (State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing) and Fengqin Dong (Institute of Botany, Chinese Academy of Sciences, Beijing) for their technical assistance for ultrathin sections preparation. We also thank Hang Su (Institute of Botany, Chi-nese Academy of Sciences, Beijing) and Wei Fan (State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing) for their technical assistance for UPLC-MS/MS analysis.

语种：英文

外文关键词：Populus; MYB10; Secondary cell wall biosynthesis; Anthocyanin biosynthesis

摘要：Secondary cell wall (SCW) formation is regulated by a multilevel transcriptional regulatory network, in which MYB transcription factors (TFs) play key roles. In woody plants, hundreds of MYB TFs have been identified, most of which have unknown functions in wood SCW biosynthesis. Here, we characterized the function of a Populus MYB gene, PtoMYB10. PtoMYB10 was found to encode an R2R3-MYB TF and exhibit dominant expression in xylem tissues. PtoMYB10 was determined to be located in the nucleus with the ability to activate transcription. Overexpression of PtoMYB10 in Populus resulted in a drastic increase in SCW thickening in xylem fiber cells as well as ectopic deposition of lignin in cortex cells. The expression of genes associated with lignin biosynthesis was induced in PtoMYB10 overexpressing plants, whereas repressed gene expression was found with the anthocyanin biosynthesis pathway. Lignin and anthocyanin are both produced from metabolites of the phe-nylpropanoid pathway. Accordingly, the anthocyanin content of Populus overexpressing PtoMYB10 decreased by more than 68%. These results indicate that PtoMYB10 can positively regulate xylary fiber SCW thickening, accompanied by the reprogramming of phenylpropanoid metabolism, which redirects metabolic flux from anthocyanin biosynthesis to monolignol biosynthesis.