文献类型：期刊文献

英文题名：Plant regeneration of Picea asperata Mast. by somatic embryogenesis

作者：Xia, Yan[1] Zhang, Jianwei[1] Jing, Danlong[1] Kong, Lisheng[2] Zhang, Shougong[1] Wang, Junhui[1]

第一作者：Xia, Yan

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing, Peoples R China;[2]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC, Canada

年份：2017

卷号：31

期号：1

起止页码：299-312

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000394283900025)】；

基金：This study was supported by a grant from the China Twelfth Five-Year Plan for Science Technology Support (2012BAD01B01). The authors acknowledge Xiao Long Shan Forestry Research Institute for cone collection and thank Prof. Iain Charles Bruce (Peking University, China) for critical reading of the manuscript.

语种：英文

外文关键词：Spruce; Embryonal masses; Somatic embryo maturation; Somatic embryo germination; Partial desiccation; Indicator

摘要：This study provides a novel desiccation indicator for somatic embryos and an efficient system for somatic seedling production in Picea asperata Mast. Picea asperata Mast. is a widely distributed native spruce in China with outstanding wood properties and adaptability. In this study, we established an effective system to propagate plantlets through somatic embryogenesis in P. asperata. The effects of cone collection date, seed family, and plant growth regulators on the initiation of embryonal masses (EM) were analyzed. Besides the significant effects of seed family and cone collection date on EM initiation, the highest induction frequency was obtained with modified Litvay medium (MLV) containing 10 A mu M 2,4-dichlorophenoxyacetic acid and 5 A mu M 6-benzylaminopurine. The best tissue proliferation in suspension cultures was obtained with an inoculum density of 1:7 (v/v, sedimented cells:liquid medium) after 9 days in culture. The best medium for somatic embryo (SE) maturation was MLV supplemented with 105 A mu M (+/-)-abscisic acid, 5% polyethylene glycol 4000, 3% sucrose, 0.1% activated charcoal (AC), 0.05% l-glutamine, 0.1% casein acid hydrolysate, and 0.4% gellan gum. In this medium, 415 +/- 103 SEs with well-developed cotyledons were obtained per gram (fresh weight) of EM. After partial desiccation treatment under low light intensity, somatic embryos, depending on their color change, were divided into green cotyledonary embryos (GCEs) and non-green cotyledonary embryos (NGCEs). These embryos, along with non-desiccated SEs as controls, were germinated on 1/2 MLV supplemented with 2% sucrose, 0.2% AC, 0.05% l-glutamine, and 0.6% gellan gum. Only GCEs, with red radicles, produced plantlets at the highest rates (> 74.5%) among all of the treatments. This efficient system of somatic seedling production in P. asperata includes screened culture factors and a novel desiccation indicator to ensure SE quality before germination.