文献类型：期刊文献

英文题名：Genome-Wide Identification and Analysis of MicroRNAs Involved in Witches'-Broom Phytoplasma Response in Ziziphus jujuba

作者：Shao, Fenjuan[1] Zhang, Qian[1] Liu, Hongwei[1] Lu, Shanfa[2,3] Qiu, Deyou[1]

第一作者：邵芬娟

通信作者：Qiu, DY[1];Lu, SF[2];Lu, SF[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Med Sci, Inst Med Plant Dev, Beijing, Peoples R China;[3]Peking Union Med Coll, Beijing, Peoples R China

年份：2016

卷号：11

期号：11

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000387615200062)】；

基金：The work was supported by the National Key Research and Development Program of China (grant number 2016YFD0600104) and the Basic Research Fund of RIF (grant number RIF2014-01).

语种：英文

摘要：MicroRNAs (miRNAs) play an important role in responding to biotic and abiotic stresses in plants. Jujube witches'-broom a phytoplasma disease of Ziziphus jujuba is prevalent in China and is a serious problem to the industry. However, the molecular mechanism of the disease is poorly understood. In this study, genome-wide identification and analysis of microRNAs in response to witches'-broom was performed. A total of 85 conserved miRNA unique sequences belonging to 32 miRNA families and 24 novel miRNA unique sequences, including their complementary miRNA* strands were identified from small RNA libraries derived from a uninfected and witches'-broom infected Z. jujuba plant. Differentially expressed miRNAs associated with Jujube witches'-broom disease were investigated between the two libraries, and 12 up-regulated miRNAs and 10 down-regulated miRNAs identified with more than 2 fold changes. Additionally, 40 target genes of 85 conserved miRNAs and 49 target genes of 24 novel miRNAs were predicted and their putative functions assigned. Using the modified 5'-RACE method, we confirmed that SPL and MYB were cleaved by miR156 and miR159, respectively. Our results provide insight into the molecular mechanisms of witches'-broom disease in Z. jujuba.