文献类型：期刊文献

英文题名：The role of plasma membrane H+-ATPase in jasmonate-induced ion fluxes and stomatal closure in Arabidopsis thaliana

作者：Yan, Suli[1,2] McLamore, Eric S.[3] Dong, Shanshan[1,2] Gao, Haibo[4] Taguchi, Masashige[3] Wang, Ningning[1,2] Zhang, Ting[1,2] Su, Xiaohua[5] Shen, Yingbai[1,2]

第一作者：Yan, Suli

通信作者：Shen, YB[1]|[a0005af9bc02893cd3fd3]沈应柏;

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China;[2]Beijing Forestry Univ, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[3]Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA;[4]Linyi Univ, Coll Life Sci, Linyi 276005, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2015

卷号：83

期号：4

起止页码：638-649

外文期刊名：PLANT JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000359356300006)】；

基金：We thank Professor Daoxin Xie for providing seeds of COI1 mutations coi1-1. We thank Dr Melanie Correll (University of Florida, USA) for her professional advice and review of the manuscript. This work was financially supported by the National Nature Science Foundation of China (31270655) and the National '863' Plant Project (no. 2011AA10020102). None of the authors have any conflict of interest.

语种：英文

外文关键词：MeJA; plasma membrane H+-ATPase; ion flux; guard cell; Arabidopsis thaliana

摘要：Methyl jasmonate (MeJA) elicits stomatal closure in many plant species. Stomatal closure is accompanied by large ion fluxes across the plasma membrane (PM). Here, we recorded the transmembrane ion fluxes of H+, Ca2+ and K+ in guard cells of wild-type (Col-0) Arabidopsis, the CORONATINE INSENSITIVE1 (COI1) mutant coi1-1 and the PM H+-ATPase mutants aha1-6 and aha1-7, using a non-invasive micro-test technique. We showed that MeJA induced transmembrane H+ efflux, Ca2+ influx and K+ efflux across the PM of Col-0 guard cells. However, this ion transport was abolished in coi1-1 guard cells, suggesting that MeJA-induced transmembrane ion flux requires COI1. Furthermore, the H+ efflux and Ca2+ influx in Col-0 guard cells was impaired by vanadate pre-treatment or PM H+-ATPase mutation, suggesting that the rapid H+ efflux mediated by PM H+-ATPases could function upstream of the Ca2+ flux. After the rapid H+ efflux, the Col-0 guard cells had a longer oscillation period than before MeJA treatment, indicating that the activity of the PM H+-ATPase was reduced. Finally, the elevation of cytosolic Ca2+ concentration and the depolarized PM drive the efflux of K+ from the cell, resulting in loss of turgor and closure of the stomata. Significance Statement Our findings provide insight into early signal transduction in guard cells in response to MeJA.