文献类型：期刊文献

英文题名：Genetic diversity and structure among natural populations of Sindora glabra in Hainan Island, China as revealed by ISSR markers

作者：Yang, Jin-chang[1] Li, Qiong-Qiong[1] Yu, Niu[1] Yin, Guang-tian[1] Wu, Zhong-feng[2] Li, Rong-sheng[1] Zou, Wen-tao[1]

第一作者：杨锦昌

通信作者：Yu, N[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Guangzhou Acad Forestry & Landscape Gardening, Guangzhou 510405, Guangdong, Peoples R China

年份：2016

卷号：69

起止页码：145-151

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390497300020)】；

基金：This work was supported by Forestry Industry Research Special Funds for Public Welfare Projects (201304604) and Fundamental Research Funds for Research Institute of Tropical Forestry (RITFYWZX201206). We thank Dr. Khongsak Pinyopusarerk for valuable comments on the draft manuscript.

语种：英文

外文关键词：Sindora glabra; ISSR; Genetic diversity; Conservation strategy

摘要：Sindora glabra, one of the second-class national protective plants in China, has important ecological and commercial values. To understand the genetic diversity and structure of S. glabra, eleven natural populations from four areas of Hainan Island in China were analyzed by inter -simple sequence repeat (ISSR) markers. Thirteen primers were screened out and used to amplify 157 DNA samples. A total of 122 bands were obtained, among which 114 (93.4%) bands were polymorphic. Genetic parameters including average number of effective alleles (1.547), Nei's gene diversity (0.321), Shannon's information index (0.482), and gene differentiation coefficient (0.1944) revealed a high level of genetic diversity maintained in S. glabra populations. The variation within populations accounted for 85.6% of total variation based on analysis of molecular variance. Genetic distance analysis showed that 11 populations could be divided into three groups and populations from the same areas were classified as one group, suggesting that high genetic diversity of S. glabra was attributable to geographic isolation. Together, introduction of germplasm from distant natural distribution areas would be a sound strategy for germplasm resource conservation of S. glabra and selection of elite individuals from populations of far relationship for hybridization is of great importance for breeding improvement programs in future. (C) 2016 Elsevier Ltd. All rights reserved.