文献类型：期刊文献

中文题名：Overexpression of CfPIP1-1,CfPIP1-2,and CfPIP1-4 genes of Catalpa fargesii in transgenic Arabidopsis thaliana under drought stress

作者：Wenjun Ma[1] Yao Xiao[1] Yuan Li[1,3] Pan Hu[1,2] Zhi Wang[1] Guijuan Yang[1] Junhui Wang[1]

第一作者：麻文俊

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,National Forest Genetic Resource Platform,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China;[2]Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,People’s Republic of China;[3]Central South University of Forestry and Technology,Changsha 410004,People’s Republic of China

年份：2021

卷号：32

期号：1

起止页码：285-296

中文期刊名：林业研究：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；

基金：Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2014QA004).

语种：英文

中文关键词：Catalpa fargesii;Drought tolerance;PIP1;Aquaporin;Heterologous transformation

分类号：S792.99

摘要：Catalpa fargesii is an important economic tree species used for furniture and timber production because of its high density and hardness.Its survival and growth are severely aff ected and primarily limited by drought stress.Thus,to better understand the mechanism of drought resistance in C.fargesii,we used qRT-PCR to reveal signifi cantly diff erent expression of three plasma membrane intrinsic protein genes:CfPIP1-1,CfPIP1-2 and CfPIP1-4.We then cloned their full-length cDNA sequences and characterized the encoded proteins.We analyzed the genes phylogenetically and predicted conserved motifs,domains,and secondary and tertiary structures.To verify the function ofthe CfPIP1 genes further,we ectopically expressed CfPIP1 transgenes in Arabidopsis thaliana.The results showed that CfPIP1-1,CfPIP1-2 and CfPIP1-4 had several characteristics of aquaporins.The transgenic plants grew better than the WT plants did under drought stress,and overexpression of the CfPIP1 genes increased the plant water content and resistance to drought.Thus,CfPIP1-1,CfPIP1-2 and CfPIP1-4 of C.fargesii play key roles in regulating the intracellular and extracellular water balance and in mediating the plant response to drought.