文献类型：期刊文献

英文题名：Changes in methane oxidation ability and methanotrophic community composition across different climatic zones

作者：Zeng, Lile[1,2,3] Tian, Jianqing[4] Chen, Huai[1,2] Wu, Ning[1,2] Yan, Zhiying[5] Du, Linfang[6] Shen, Yan[7] Wang, Xu[8]

第一作者：Zeng, Lile

通信作者：Chen, H[1];Chen, H[2];Tian, JQ[3]

机构：[1]Chinese Acad Sci, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu 610041, Sichuan, Peoples R China;[2]Chinese Acad Sci, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;[3]Hubei Jingzhou Ancient City Ring Natl Wetland Pk, Jingzhou 434000, Peoples R China;[4]Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China;[5]Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China;[6]Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China;[7]Cent South Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China;[8]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2019

卷号：19

期号：2

起止页码：533-543

外文期刊名：JOURNAL OF SOILS AND SEDIMENTS

收录：;Scopus(收录号：2-s2.0-85049136936);WOS:【SCI-EXPANDED(收录号:WOS:000457745700003)】；

基金：This work was supported by the National Key R&D Program of China (Grant No. 2016YFC0501804), the 1000 Talents Program of Sichuan Province of China, and the Youth Science and Technological Innovation Team Program, Sichuan Province (Grant No. 2015TD0026).

语种：英文

外文关键词：Aerobic methane-oxidizing bacteria; Methanotroph composition; Subtropical forest; Temperate forest

摘要：PurposeMicrobial oxidation by bacteria with the potential to oxidize C1 compounds (methanotrophs) is the only biological sink for atmospheric methane (CH4). Aerobic methanotrophs are particularly active in forest soils, but the role of aerobic methanotrophs in native forest soils in China remains poorly understood. The pmoA gene, encoding the key enzyme methane monooxygenase (particulate MMO), is widely used to identify methanotrophic communities.Materials and methodsWe collected soils from different vegetation types in one subtropical and one temperate forest in China. Potential CH4 oxidation rates and methanotroph communities were assessed via laboratory incubation and pmoA-based phylogenetic analysis, respectively.Results and discussionAcross all sampling sites, we observed distinct variations in methanotroph community composition and CH4 oxidation rates. In all soils, CH4 oxidation rates increased with increasing CH4 concentration. Elevated temperature resulted in an increase in the CH4 oxidation rates in coniferous forests, while a decrease in deciduous forests. Restriction fragment length polymorphism analyses indicated that methantrophic community varied in different vegetation types. The methanotroph communities were dominated by type II methanotrophs (including soil cluster alpha (SC), Methylocystis, and USC) and type I methanotrophs (including USC and Methylobacter) in deciduous and coniferous forests, respectively.ConclusionsIt is suggested that intrinsic differences in CH4 oxidation rate responses to temperature between coniferous and deciduous soils are likely due to different methanotroph community structures. Taken together, the direction of CH4 feedback responses to disturbance was site specific.