文献类型：期刊文献

英文题名：A chromosome-scale genome of Hippophae neurocarpa provides new insights into serotonin biosynthesis and chlorophyll-derived brown fruit coloration

作者：Chen, Ning[1,2] Zhang, Guo-yun[1,2] Song, Ya-ting[1,2] Yang, Yang[1,2] Zhang, Jian-guo[1,2,3] He, Cai-yun[1,2]

第一作者：Chen, Ning

通信作者：Zhang, GY[1];Zhang, JG[1];He, CY[1];Zhang, GY[2];Zhang, JG[2];He, CY[2];Zhang, JG[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Res Inst Forestry, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing, Peoples R China

年份：2025

卷号：121

期号：5

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20251018005472);Scopus(收录号：2-s2.0-85219750359);WOS:【SCI-EXPANDED(收录号:WOS:001442843400001)】；

基金：This work was supported by the Fundamental Research Funds of CAF (CAFYBB2024QG007), the National Natural Science Foundation of China (32261133521), and the Science and Technology Development Center of the National Forestry and Grassland Administration (KJZXSA202404).

语种：英文

外文关键词：Hippophae neurocarpa; chromosomal level genome assembly; phylogenetics; serotonin biosynthesis; fruit coloration

摘要：Sea buckthorn (Hippophae neurocarpa), a plant with both medicinal and edible properties, exhibits high content of serotonin with a unique brown color. Here, we assemble the 1002.54 Mb genome sequence of H. neurocarpa and find that it has evolved from two sequential polyploidizations with four sub-genomes. Based on in vitro enzyme activity and transient overexpression experiments, we confirm that the expression of both HnT5H1 and HnT5H2 genes from tandem duplication contributes to high accumulation of serotonin in sea buckthorn. Furthermore, we verify that the HnGLK1 gene plays a crucial role in continuous chlorophyll accumulation, driving the brown color formation of H. neurocarpa fruit. Collectively, the high-quality genome of H. neurocarpa offers valuable insights into the mechanisms underlying serotonin biosynthesis and abnormal coloration and serves as a valuable resource for further functional genomic studies and molecular breeding efforts in sea buckthorn.