文献类型：期刊文献

英文题名：Effect of Salicylic Acid on the Gene Transcript and Protein Accumulation of Flavonoid Biosynthesis-related Enzymes in Vitis vinifera Cell Suspension Cultures

作者：Wang, Huiling[1,2] Wang, Wei[3] Huang, Weidong[4] Xu, Haiying[1,5]

第一作者：Wang, Huiling

通信作者：Xu, HY[1];Huang, WD[2];Xu, HY[3]

机构：[1]Beijing Acad Forestry & Pomol Sci, Beijing 100097, Peoples R China;[2]Minist Agr, Key Lab Urban Agr North China, Beijing, Peoples R China;[3]Chinese Acad Forestry, Key Lab Silviculture, State Forestry Adm, Inst Forestry, Beijing 100091, Peoples R China;[4]China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China;[5]Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100097, Peoples R China

年份：2017

卷号：52

期号：12

起止页码：1772-+

外文期刊名：HORTSCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000422982200021)】；

基金：This research was supported by the special fund of the China Agriculture Research System (CARS-29), the National Natural Science Foundation of China (grant number 31601712), and the Natural Science Foundation of Beijing Academy of Agriculture and Forestry Sciences (No. QNJJ201604).

语种：英文

外文关键词：grape; anthocyanins; proanthocyanidins; paclobutrazol; protein accumulation

摘要：Salicylic acid (SA) as a plant signal molecule plays an important regulatory role in stimulating the accumulation of plants' defensive compounds. Numerous studies have shown its regulating effects on the biosynthesis of flavonoids. However, the controlling mechanism needs to be made clear further. Here, Vitis vinifera L. 'Cabernet Sauvignon' cell suspension cultures were treated with SA and its synthesis inhibitor paclobutrazol (Pac) to investigate their effects on flavonoid biosynthesis. The accumulation of gene transcripts and proteins of flavonoid biosynthesis pathway enzymes, including chalcone synthase (VvCHS), chalcone isomerase (VvCHI), dihydroflavonol 4-reductase (VvDFR), and anthocyanidin synthase (VvANS), were detected using real-time polymerase chain reaction (RT-PCR), quantitative polymerase chain reaction (qPCR), and westernblotting techniques. The results showed that treatment with exogenous SA at the proper concentration enhanced the production of flavonoids such as anthocyanins and proanthocyanidins (PAs) inside the suspension-cultured cells. The induction of anthocyanins and PAs was found to be time course-dependent. The mRNA and protein accumulation of VvCHS, VvCHI, VvDFR, and VvANS in the treatment system were enhanced too, and the changes were observed concomitantly. Treatment of Pac inhibited the induction of the accumulation of gene transcripts and proteins in accordance with the decreased accumulation of flavonoids. These results suggest that exogenous SA could induce both transcript and protein accumulation of flavonoid biosynthesis-related enzymes and in turn enhance the accumulation of flavonoid compounds such as anthocyanins and PAs in suspension-cultured grape cells.