文献类型：期刊文献

英文题名：Functional analyses of a flavonol synthase-like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation

作者：Zhou, Xing-Wen[1,2] Fan, Zheng-Qi[1] Chen, Yue[1] Zhu, Yu-Lin[2] Li, Ji-Yuan[1] Yin, Heng-Fu[1]

第一作者：Zhou, Xing-Wen

通信作者：Yin, HF[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[2]Yulin Normal Univ, Yulin 537000, Guangxi, Peoples R China

年份：2013

卷号：38

期号：3

起止页码：593-604

外文期刊名：JOURNAL OF BIOSCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000323854800015)】；

基金：This work was supported by the funds from Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period (NO.2012BAD01B0703). We also acknowledge International Science & Technology Cooperation Program of China (2011DFA30490), Breeding New Flower Varieties Program of Zhejiang Province (2012C12909-6), the CAF Nonprofit Research Projects (RISF6141), and National Science Foundation of Guangxi Region (2012GXNSFBA053077). We greatly appreciate the two anonymous reviewers for their critical reading and helpful comments.

语种：英文

外文关键词：Anthocyanin; Camellia; flavonoid; flavonol synthase; flavonol; pigmentation

摘要：The flavonoids metabolic pathway plays central roles in floral coloration, in which anthocyanins and flavonols are derived from common precursors, dihydroflavonols. Flavonol synthase (FLS) catalyses dihydroflavonols into flavonols, which presents a key branch of anthocyanins biosynthesis. The yellow flower of Camellia nitidissima Chi. is a unique feature within the genus Camellia, which makes it a precious resource for breeding yellow camellia varieties. In this work, we characterized the secondary metabolites of pigments during floral development of C. nitidissima and revealed that accumulation of flavonols correlates with floral coloration. We first isolated CnFLS1 and showed that it is a FLS of C. nitidissima by gene family analysis. Second, expression analysis during floral development and different floral organs indicated that the expression level of CnFLS1 was regulated by developmental cues, which was in agreement with the accumulating pattern of flavonols. Furthermore, over-expression of CnFLS1 in Nicotiana tabacum altered floral colour into white or light yellow, and metabolic analysis showed significant increasing of flavonols and reducing of anthocyanins in transgenic plants. Our work suggested CnFLS1 plays critical roles in yellow colour pigmentation and is potentially a key point of genetic engineering toward colour modification in Camellia.