文献类型：期刊文献

英文题名：Reference gene selection for qRT-PCR in Caragana korshinskii Kom. under different stress conditions

作者：Yang, Qi[1] Yin, Jiajia[1] Li, Gao[1] Qi, Liwang[2] Yang, Feiyun[3] Wang, Ruigang[1] Li, Guojing[1]

第一作者：Yang, Qi

通信作者：Li, GJ[1]

机构：[1]Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Inner Mongolia Agr Univ, Coll Food Sci & Bioengn, Hohhot 010018, Peoples R China

年份：2014

卷号：41

期号：4

起止页码：2325-2334

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000333707100048)】；

基金：We deeply appreciate Dr. Mark Goettel, Editor-in-Chief of Biocontrol Science & Technology, for polishing the manuscript carefully. This work was financially supported by National Natural Science Foundation of China (No. 31360169), Chinese National Programs for High Technology Research and Development (No. 2011AA100203), the Program for New Century Excellent Talents of University (No. ECNT-11-1020) from Ministry of Education (China), and the Innovative Research Group Fund (No. NDPYTD2010-3) from Inner Mongolia Agricultural University (China).

语种：英文

外文关键词：Caragana korshinskii Kom.; qRT-PCR; Reference gene; Stress conditions

摘要：Caragana korshinskii Kom., which is widely distributed in the northwest China and Mongolia, is an important forage bush belonging to the legume family with high economic and ecological value. Strong tolerance ability to various stresses makes C. korshinskii Kom. a valuable species for plant stress research. In this study, suitable reference genes for quantitative real-time reverse transcription PCR (qRT-PCR) were screened from 11 candidate reference genes, including ACT, GAPDH, EF1 alpha, UBQ, TUA, CAP, TUB, TUB3, SKIP1, SKIP5-1 and SKIP5-2. A total of 129 samples under drought, heat, cold, salt, ABA and high pH treatment were profiled, and software such as geNORM, NormFinder and BestKeeper were used for reference gene evaluation and selection. Different suitable reference genes were selected under different stresses. Across all 129 samples, GAPDH, EF1 alpha and SKIP5-1 were found to be the most stable reference genes, and EF1 alpha+SKIP5-1 is the most stable reference gene combination. Conversely, TUA, TUB and SKIP1 were not suitable for using as reference genes owing to their great expression variation under some stress conditions. The relative expression levels of CkWRKY1 were detected using the stable and unstable reference genes and their applicability was confirmed. These results provide some stable reference genes and reference gene combinations for qRT-PCR under different stresses in C. korshinskii Kom. for future research work, and indicate that CkWRKY1 plays essential roles in response to stresses in C. korshinskii.