文献类型：期刊文献

英文题名：Characterization of Brassinazole resistant (BZR) gene family and stress induced expression in Eucalyptus grandis

作者：Fan, Chunjie[1,2] Guo, Guangsheng[1] Yan, Huifang[1,3] Qiu, Zhenfei[1] Liu, Qianyu[1] Zeng, Bingshan[1]

第一作者：范春节

通信作者：Zeng, BS[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

年份：2018

卷号：24

期号：5

起止页码：821-831

外文期刊名：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000442459400009)】；

基金：We gratefully acknowledge National Natural Science Foundation of China (Grant Number: 31400554) and the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (Grant Noumber: CAFYBB2014QB040).

语种：英文

外文关键词：Brassinosteroids; BZR; Eucalyptus; Abiotic stress; Gene expression

摘要：Brassinosteroids (BRs) are a group of plant hormones which play a pivotal role in modulating cell elongation, stress responses, vascular differentiation and senescence. In response to BRs, BRASSINAZOLE-RESISTANT (BZR) transcription factors (TFs) accumulate in the nucleus, where they modulate thousands of target genes and coordinate many biological processes, especially in regulating defense against biotic and abiotic stresses. In this study, 6 BZR TFs of Eucalyptus grandis (EgrBZR) from a genome-wide survey were characterized by sequence analysis and expression profiling against several abiotic stresses. The results showed that BZR gene family in Eucalyptus was slightly smaller compared to Populus and Arabidopsis, but all phylogenetic groups were represented. Various systematic in silico analysis of these TFs validated the basic properties of BZRs, whereas comparative studies showed a high degree of similarity with recognized BZRs of other plant species. In the organ-specific expression analyses, 4 EgrBZRs were expressed in vascular tissue indicating their possible functions in wood formation. Meanwhile, almost all EgrBZR genes showed differential transcript abundance levels in response to exogenously applied BR, MeJA, and SA, and salt and cold stresses. Besides, protein interaction analysis showed that all EgrBZR genes were associated with BR signaling directly or indirectly. These TFs were proposed as transcriptional activators or repressors of abiotic stress response and growth and development pathways of E. grandis by participating in BR signaling processes. These findings would be helpful in resolving the regulatory mechanism of EgrBZRs in stress resistance conditions but require further functional study of these potential TFs in Eucalyptus.