文献类型：期刊文献

英文题名：ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation.

作者：Wang, Meizhen[1] Deng, Yuxing[1] Shao, Fenjuan[2] Liu, Miaomiao[1] Pang, Yongqi[1] Li, Caili[1] Lu, Shanfa[3]

第一作者：Wang, Meizhen

机构：[1]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing, 100193, China.;[2]State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.;[3]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing, 100193, China. sflu@implad.ac.cn.

年份：2017

卷号：1640

外文期刊名：Methods in molecular biology (Clifton, N.J.)

收录：PubMed(收录号：28608342)

语种：英文

外文关键词：ARGONAUTE;Gene identification;Genetic transformation;Salvia miltiorrhiza;Small RNA

摘要：Small RNA-mediated gene silencing is a vital regulatory mechanism in eukaryotes that requires ARGONAUTE (AGO) proteins. Salvia miltiorrhiza is a well-known traditional Chinese medicinal plant. Therefore, it is important to characterize S. miltiorrhiza AGO family genes as they may be involved in multiple metabolic pathways. This chapter introduces the detailed protocol for SmAGO gene prediction and molecular cloning. In addition, an Agrobacterium-mediated genetic transformation method for S. miltiorrhiza is presented. These methodologies can be used to functionally study SmAGO genes as well as other genes of interest in S. miltiorrhiza.