文献类型：期刊文献

英文题名：Transcriptome-wide effect ofSalix SmSPR1in etiolated seedling ofArabidopsis

作者：Liu, Xiaoxia[1,3] Zhang, Jianguo[1,2,3] Xue, Li[1,3] Rao, Guodong[1,3]

通信作者：Zhang, JG[1];Rao, GD[1];Zhang, JG[2];Zhang, JG[3];Rao, GD[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 100091, Peoples R China

年份：2021

卷号：32

期号：3

起止页码：975-985

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000562366400001)】；

基金：Project funding: This work was supported by The Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2018QB001).

语种：英文

外文关键词：Arabidopsis thaliana; Cell anisotropism; SmSPR1; Transcription factor; Transcriptome analysis

摘要：Microtubules and their regulatory proteins are involved in the regulation of plant cell morphology. SPIRAL1 (SPR1), a plant-specific microtubule-binding protein, is critical in regulating the anisotropic growth of plant cells. Our previous study showed that overexpressedSalix SmSPR1genes inArabidopsis thalianacaused right-handed spiral elongation in etiolated seedlings, but there were no morphological differences between wild-type and transgenic seedlings under varied light conditions. We then studied the transcriptional regulation patterns in transgenic plants engineered with theSmSPR1gene. Transcriptomic results showed that a large number of differentially expressed genes were involved in plant light signal reception, chlorophyll synthesis and photosystem structure. Eleven gene families with 42 photosynthesis-related genes and 6 light-responsive genes were involved in regulation of cell morphology. Our results showed that these genes in theSmSPR1-oxline were particularly down-regulated under dark conditions. In addition, 33 TFs showed differences betweenSmSPR1-oxand wild-type lines. Taken together, the transcriptome analysis provides new insight into investigating the molecular mechanisms of light-induced cell morphological changes mediated by the microtubule binding protein SPR1.