文献类型：期刊文献

中文题名：蛇皮果基因组DNA提取及其RAPD条件优化

英文题名：DNA extraction and RAPD Condition optimization for Salacca edulis Reinw

作者：李建光[1] 李荣生[1] 李发根[1] 尹光天[1] 杨锦昌[1] 邹文涛[1]

第一作者：李建光

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：32

期号：6

起止页码：110-114

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：中央级公益性科研院所基本科研业务费专项项目"蛇皮果栽后遮荫和灌溉技术研究"(编号:RITFKYYW2010-02);国家林业局948引进项目"蛇皮果栽培品种及配套栽培技术引进"(编号:2007-4-11)

语种：中文

中文关键词：蛇皮果;DNA提取;RAPD优化

外文关键词：Salacca edulis; salak; DNA extraction; RAPD optimization

分类号：S789.5

摘要：以蛇皮果顶梢嫩叶为材料,比较了CTAB法、改良CTAB法、CTAB-free法和SDS法对基因组DNA的提取效果,并对蛇皮果RAPD反应体系进行优化。结果表明,CTAB法、改良CTAB法和CTAB-free法均能提取出蛇皮果基因组DNA,其中NaCl浓度为5 m ol/L的改良CTAB法因可更有效地去除多糖及酚类物质,并能满足PCR扩增的要求而被认为是蛇皮果基因组DNA提取的最适方法;在15μL的PCR扩增体系中,Mg2+、dNTPs和Taq DNA聚合酶的最优浓度分别为1.4 mmol/L、0.12 mmol/L和0.12 U/μL;PC R反应程序的最适退火温度为39℃。
By taking the tender leaves on top-shoots of Salacca edulis as tested materials, the extraction effects of CTAB, improved CTAB, CTAB-free and SDS on the genomic DNA of salak were investigated and compared. And the reaction system of salak RAPD was optimized. The results show that CTAB, improved CTAB, CTAB-free all could extracted out genomic DNA from salak, of them, the improved CTAB with NaC1 concentration of 5 mol/L could more effectively remove polysaccharides and polyphenols than the other two methods, and meet the requirement of PCR amplification, so it is the best method. The optimal concentrations of Mg2＋, dNTPs, Taq DNA polymerase and the annealing temperature are 1.4 mmol/L, 0.12 mmol/L, 0.12 U/μL and 39 ℃ respectively for the PCR progress for salak.