文献类型：期刊文献

中文题名：凤阳山毛竹入侵杉木林土壤细菌群落结构分析

英文题名：Effects of Phyllostachys edulis Invasion on Soil Bacterial Community Structure in Cunninghamia lanceolata Forest

作者：彭辉[1] 陈焕伟[2] 李求洁[3] 童冉[4] 朱念福[4] 周红敏[2]

第一作者：彭辉

机构：[1]百山祖国家公园龙泉保护中心,浙江龙泉323700;[2]龙泉市林业科学研究院,浙江龙泉323700;[3]龙泉市林业局,浙江龙泉323700;[4]中国林业科学研究院亚热带林业研究所,浙江杭州311400

年份：2024

卷号：44

期号：6

起止页码：8-15

中文期刊名：浙江林业科技

外文期刊名：Journal of Zhejiang Forestry Science and Technology

基金：“尖兵”“领雁”研发攻关计划(2022C02053);浙江省林业省院合作重大项目(2020SY04)。

语种：中文

中文关键词：凤阳山;高通量测序;土壤细菌;毛竹入侵;杉木林

外文关键词：Fengyang Mountain;Illumina MiSeq;soil bacterial;Phyllostachys edulis invasion;Cunninghamia lanceolata forest

分类号：S718.81

摘要：【目的】分析毛竹Phyllostachys edulis入侵杉木Cunninghamia lanceolata林后的细菌群落结构特征,揭示毛竹入侵杉木林对土壤细菌群落结构与林分及土壤环境因子之间的关系。【方法】采用高通量测序技术分析毛竹入侵杉木林后土壤细菌群落结构及其与土壤理化因子的相关关系。【结果】3种林型土壤共获得36门108纲241目354科552属2939个OTU土壤细菌。3种林型土壤中的优势菌门为酸杆菌门Acidobacteria、变形菌门Proteobacteria和绿弯菌门Chloroflexi,3个菌门的相对丰度占总量的71.15%~76.07%。杉木纯林和杉木毛竹混交林土壤酸杆菌门的相对丰度显著高于毛竹纯林土壤酸杆菌门的相对丰度(P<0.05),毛竹纯林和杉木毛竹混交林土壤中变形菌门的相对丰度显著高于杉木纯林土壤中变形菌门的相对丰度(P<0.05),杉木纯林土壤中绿弯菌门的相对丰度显著高于毛竹纯林土壤中绿弯菌门的相对丰度(P<0.05)。从3种林型中鉴别出的相对丰度均大于1%的细菌纲为酸杆菌纲Acidobacteriae、α-变形菌纲Alphaproteobacteria、纤线杆菌纲Ktedonobacteria、浮霉菌纲Planctomycetes、γ-变形菌纲Gammaproteobacteria、放线菌纲Actinobacteria、嗜热油菌纲Thermoleophilia、Vicinamibacteria和酸微菌纲Acidimicrobiia。优势菌纲为酸杆菌纲和α-变形菌纲。3种林型中鉴别出的相对丰度在1%以上的细菌属为HSB_OF53-F07、热酸菌属Acidothermus、念珠菌固体杆菌属Candidatus_Solibacter、酸杆菌属Acidibacter、Bryobacter和慢生根瘤菌属Bradyrhizobium。优势菌属和土壤环境因子的Spearman相关性分析表明,土壤含水率和有效磷含量是影响土壤细菌群落的主要因子。【结论】毛竹入侵杉木林后对土壤细菌群落有显著影响,杉木毛竹混交林和毛竹纯林土壤中单独出现的非优势菌属可为研究毛竹入侵杉木林过程中土壤细菌群落的变化提供重要参考。
【Objective】This paper aims to elucidate the impacts of soil bacterial community structure after Phyllostachys edulis invaded Cunninghamia lanceolata forest,and reveals the relationship between bacterial community diversity and structure and forest types as well as soil environment factors.【Method】Illumina MiSeq high throughput sequencing and OTU analysis were used to assess the soil bacterial community structure and its correlation with soil physical and chemical factors in Cunninghamia lanceolata forest invaded by Phyllostachys edulis(CP)to compare with that in Phyllostachys edulis forest(PF)and Cunninghamia lanceolata forest(CF).【Result】A total of 2939 OTU soil bacteria of 36 phyla,108 classes,241 orders,354 families,552 genera were obtained from this 3 forest stand samples,among them Acidobacteria,Proteobacteria,Chloroflexi were the predominant bacterial phyla,accounting for 71.15%-76.07%.The relative abundance of Acidobacteria in CF and CP was significantly higher than that in PF(P<0.05),the relative abundance of Proteobacteria in CF was significantly lower than that in other two forests,the relative abundance of Chloroflexi in CF was significantly higher than that in PF.The bacteria with relative abundance of more than 1%identified in the 3 forest sample plots are:Acidobacteriia,Alphaproteobacteria,Ktedonobacteria,Planctomycetes,Gammaproteobacteria,Actinobacteria,Thermoleophilia,Vicinamibacteria and Acidimicrobiia,Acidobacteriia and Alphaproteobacteria are two dominant classes.The bacterial genera with relative abundance of more than 1%identified in the 3 forests are:HSB_OF53-F07,Acidothermus,Candidatus Solibacter,Acidobacterium,Bryobacter and Bradyrhizobium.Spearman correlation analysis between dominant bacterial genera and soil environmental factors showed that,soil water content and available phosphorus are the main factors affecting soil bacterial community.【Conclusion】The invasion of Phyllostachys edulis into the Cunninghamia lanceolata forest has a significant impact on the soil bacterial community.The non-dominant bacteria isolated from the CP and the PF can provide an important reference for studying the changes of bacterial community during this intrusion process.