文献类型：期刊文献

英文题名：Regulation of synchronism by abscisic-acid-responsive small noncoding RNAs during somatic embryogenesis in larch (Larix leptolepis)

作者：Zhang, Jun-Hong[1,2] Zhang, Shou-Gong[1] Li, Shui-Gen[1] Han, Su-Ying[3] Li, Wan-Feng[1] Li, Xin-Min[1,4] Qi, Li-Wang[1]

第一作者：Zhang, Jun-Hong

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing 100091, Peoples R China;[2]Zhejiang Agr & Forestry Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Key Lab Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[4]Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

年份：2014

卷号：116

期号：3

起止页码：361-370

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000331648900009)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 31330017; 31300566), and the National High Technology Research and Development Program of China (Grant Nos. 2011AA100203; 2013AA102704).

语种：英文

外文关键词：Larix leptolepis; Small RNA; MicroRNA; Synchronism; Somatic embryogenesis

摘要：Low levels of abscisic acid (ABA) are associated with embryo asynchronism during somatic embryogenesis. Small non-coding RNAs (sncRNAs) are emerging as key regulators of embryogenesis. To investigate the roles of sncRNAs in regulating the synchronism of somatic embryogenesis in Larix leptolepis, we examined the endogenous "sncRNAome" in synchronous and asynchronous embryos. Non-redundant sRNAs 24 nt in length were overrepresented in both synchronous and asynchronous embryos, while the majority of redundant sRNAs were 24 nt long in synchronous embryos and 21 nt long in asynchronous embryos. Many more non-redundant unknown sRNAs were expressed in synchronous embryos than in asynchronous embryos, indicating that many sRNAs might participate in the regulation of synchronism during larch somatic embryogenesis. However, the proportion of sRNAs that were miRNAs was higher in asynchronous embryos than in synchronous embryos, and their expression was much higher in asynchronous embryos. Although the proportion of sRNAs that matched repeat regions (ra-siRNA) was higher in asynchronous embryos than in synchronous embryos, the ratio of 24-nt ra-siRNAs to total sRNAs was much lower in asynchronous embryos than in synchronous embryos. Further analysis suggested that miRNAs exemplified by miR156 might participate in the regulation of embryo synchronism in larch, which causes a large number of transcripts encoding differentiation-promoting factors to be down regulated. Therefore, we speculate that sncRNAs induced by ABA inhibit the precocious expression of differentiation-promoting factors, thereby regulating the synchronism of somatic embryogenesis.