文献类型：期刊文献

英文题名：Genomic Analysis Reveals the Fast-Growing Trait and Improvement Potential for Stress Resistance in the Elite Poplar Variety Populus x euramericana 'Bofeng 3'

作者：Zhong, Shanchen[1,2] Zhang, Weixi[1,2] Ding, Changjun[1,2] Yuan, Zhengsai[1,2] Shen, Le[1,2,3] Zhang, Bingyu[1,2] Chu, Yanguang[1,2] Su, Xiaohua[1,2,4]

第一作者：Zhong, Shanchen

通信作者：Chu, YG[1];Su, XH[1];Chu, YG[2];Su, XH[2];Su, XH[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat State Forestry, Beijing 100091, Peoples R China;[3]Jiangxi Acad Sci, Inst Biol Resources, Jiangxi Prov Key Lab Improved Variety Breeding & E, Nanchang 330046, Peoples R China;[4]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

年份：2025

卷号：26

期号：12

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001515841800001)】；

基金：This research was funded by the National Key R&D Program of China during the 14th Five-year Plan Period (grant number 2021YFD2200103) and the Biological Breeding-National Science and Technology Major Project (grant number 2023ZD0405603).

语种：英文

外文关键词：Populus x euramericana 'Bofeng 3'; genome; transcriptome; comparative genomics; drought tolerances; salt resistance

摘要：Enhancing stress tolerance represents a critical objective in the genetic improvement of poplar trees. Populus x euramericana 'Bofeng 3' is a nationally certified elite poplar variety that was approved as a premium pulpwood variety for the southern area of Northeastern China. This variety grows quickly, has good yield, and resists frost; however, its weaker drought and salt tolerance limits its broader use in diverse environments. The aim of this study is to understand the genetic basis of the fast growth and stress-adaptation traits of this variety and to provide support for future molecular breeding efforts. We present a chromosome-scale genome assembly of Populus x euramericana 'Bofeng 3', totaling 445.53 Mb, of which with 90.39% is anchored to 19 chromosomes, containing 33,309 protein-coding genes and 45.36% repetitive elements. Comparative genomics showed that 'Bofeng 3' has expanded gene families related to photosynthesis and metabolism, and contracted families involved in stress responses, distinguishing it from other Populus species. Under drought (9137 leaf, 9403 root differentially expressed genes (DEGs)) and salt stress (2840 leaf, 3807 root DEGs), trend analysis revealed specific expression patterns. Several unique and expanded genes, including those for photosynthetic proteins, peroxidases, gamma-aminobutyric acid metabolism, and disease resistance, showed stress-responsive trends. Weighted gene co-expression network analysis identified five modules (three positive, two negative) that significantly correlated with photosynthetic traits, highlighting key candidates such as bZIP transcription factors and auxin/indole acetic acid genes. This study determined the genetic basis underlying the rapid growth traits of Populus x euramericana 'Bofeng 3', while providing genomic resources to establish a robust foundation for future gene editing and molecular breeding studies, including critical candidate genetic resources for developing superior drought- and salt-tolerant poplar varieties via targeted genome editing technologies.