文献类型：期刊文献

中文题名：硼对黑木相思幼苗生长发育的影响

英文题名：Effects of Boron on the Growth and Development of Acacia melanoxylon R.Br. seedlings

作者：陈朝黎[1] 白晓刚[1,2] 李湘阳[1] 曾炳山[1] 胡冰[1]

第一作者：陈朝黎

机构：[1]中国林业科学研究院热带林业研究所,热带林业研究国家林业和草原局重点实验室,广东广州510520;[2]仲恺农业工程学院农业与生物学院,广东广州510225

年份：2023

卷号：36

期号：6

起止页码：181-191

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：广东省林业科技创新项目(2020KJCX014);国家重点研发计划子课题(2022YFD2200205-1)。

语种：中文

中文关键词：黑木相思;硼胁迫;营养元素;主成分分析;生长发育

外文关键词：Acacia melanoxylon;boron stress;nutrient elements;principal component analysis;growth and development

分类号：S722

摘要：[目的]研究不同供硼量培养条件下,黑木相思生长指标、生理生化特性和营养元素含量的变化,揭示黑木相思对硼胁迫环境的响应模式。[方法]以1月龄黑木相思无性系SR17幼苗为材料,利用含0、0.1、1、2 mmol·L^(-1)硼酸的营养液培养2个月后,测定黑木相思的生长指标、叶绿素含量、生理活性物质含量、氧化还原酶活性和营养元素含量等生理生化指标的变化,基于主成分与隶属函数分析,综合评价各指标对黑木相思响应不同供硼量的贡献率,比较黑木相思幼苗对不同供硼量的适应性。[结果]试验结果表明,0、1和2 mmol·L^(-1)供硼量抑制了黑木相思幼苗株高和根长的增加,降低了地上部分叶绿素的含量,引起叶片失绿、黄化甚至脱落的表型。0 mmol·L^(-1)供硼量使黑木相思的主根和侧根呈白色且增粗,1、2 mmol·L^(-1)供硼量使根系褐化。0、1、2 mmol·L^(-1)供硼量降低了黑木相思体内抗坏血酸(AsA)的含量,引起了氧化反应;增加了丙二醛(MDA)和脯氨酸(Pro)含量,引起体内脂质氧化水平升高和渗透胁迫;增加了体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脂氧合酶(LOX)和苯丙氨酸解氨酶(PAL)的活性,引发了氧自由基和过氧化氢(H_(2)O_(2))的积累与清除以及次生代谢物的合成。黑木相思体内硼元素含量增加,磷和钾元素的含量也随之增加,而钙元素含量在0.1 mmol·L^(-1)供硼量下最高。通过主成分和隶属函数分析,钾元素含量、叶片数增长量、株高增长量、LOX活性、AsA含量、钙元素含量、硼元素含量、POD活性、Pro含量、SOD活性、根长增长量、蛋白浓度、PAL活性及叶绿素b含量14个指标可作为黑木相思生长发育响应硼胁迫的主要指标。[结论]黑木相思幼苗在0.1mmol·L^(-1)供硼量下生长势较好,其生长指标、生理生化特性和营养元素含量在不同供硼量条件下差异显著,黑木相思通过调控植株表型、生理活性物质含量、氧化还原酶活性和营养元素含量响应硼胁迫。
[Objective]To study the changes of growth indicators,physiological and biochemical character-istics,and nutrient element content of Acacia melanoxylon R.Br.under different boron content,and reveal the response mode of A.melanoxylon to boron stress environment.[Method]The A.melanoxylon clone SR171-month-old seedlings were cultured with nutrient solution containing 0,0.1,1,and 2 mmol·L^(-1) bor-ic acid for 2 months.The changes in the physiological and biochemical indexes,such as growth index,chlorophyll content,physiologically active substances content,oxidoreductase activity,and nutrient ele-ment content,were determined under each culture condition.Based on principal component and subordin-ate function analysis,the contribution rate of each index for A.melanoxylon response to different contribu-tion boron content was comprehensively evaluated,and the stress degree of A.melanoxylon seedlings with different boron supply levels was compared.[Result]The results showed that 0,1,and 2 mmol·L^(-1) boron supply inhibited the increase of plant height and root length,reduced the aboveground chlorophyll content,and caused leaf degreenization,yellowing,and even abruption phenotypes.0 mM boron made the primary and lateral roots white and thickened,1 and 2 mmol·L^(-1) boron made the roots brown.The dosages of 0,1,and 2 mmol·L^(-1) boron reduced the content of ascorbic acid(AsA)and caused an oxidation reaction.The contents of malondialdehyde(MDA)and proline(Pro)were increased,leading to increased lipid oxidation levels and osmotic stress.It increased the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),lipoxygenase(LOX),and phenylalanine ammonlyase(PAL)in vivo,leading to the accumu-lation and removal of oxygen-free radicals and hydrogen peroxide(H_(2)O_(2))as well as the synthesis of sec-ondary metabolites.The increases of boron content of A.melanoxylon promoted the increase of phosphor-us and potassium content,and the calcium content was the highest under 0.1 mmol·L^(-1) boron supply.Through principal component and subordinate function analysis,fourteen indexes,including potassium content,leaf number increase,plant height increase,LOX activity,AsA content,calcium content,boron content,POD activity,Pro content,SOD activity,root length increase,protein concentration,PAL activity,and chlorophyll b content,could be used as the main indexes of A.melanoxylon growth and development response to boron stress.[Conclusion]A.melanoxylon seedlings grow better under 0.1mmol·L^(-1) boron supply,and their growth indicators,physiological and biochemical characteristics,and nutrient element content are significantly different under different boron supply conditions.A.melanoxylon responds to boron stress by regulating plant phenotype,the content of physiologically active substances,oxidoreductase activity,and nutrient element content.