文献类型：期刊文献

中文题名：木本植物非均质化组织石蜡切片制作方法

英文题名：New Protocols for Paraffin Sections of Heterogeneous Tissues of Woody Plants

作者：周乃富[1] 张俊佩[1] 刘昊[2] 查巍巍[1] 裴东[1]

第一作者：周乃富

机构：[1]中国林业科学研究院林业研究所林木遗传育种国家重点实验室国家林业局林木培育重点实验室;[2]天津市林业果树研究所

年份：2018

卷号：53

期号：5

起止页码：653-660

中文期刊名：植物学报

外文期刊名：Chinese Bulletin of Botany

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：燕山特色坚果新品种耐旱丰产技术研究集成与科技示范(No.Z161100000916011);中国核桃优异种质遗传信息评价(No.RIF-2013-01)

语种：中文

中文关键词：非均质化组织;改良方案;核桃;石蜡切片

外文关键词：heterogeneous plant tissue;improved protocol;Juglans regia;paraffin section

分类号：S685.12

摘要：在常规石蜡切片技术的基础上,针对木本植物茎段木质化程度高、硬度大以及各部分组织硬度不均匀等特点,选取核桃(Juglans regia)茎段以及芽接愈合区域组织为实验材料,对固定、软化和脱水等关键步骤进行改进,获得结构完整且染色清晰的茎段组织和嫁接愈合区域组织切片,可清晰地观察到各部分组织的形态特征和愈合过程中的发育特征,且缩短了制片周期。采用改良后的实验流程成功获得了苹果(Malus pumila)、桃(Amygdalus persica)、杏(Armeniaca vulgaris)、李(Prunus salicina)和杨(Populus tomentosa)的茎段横截面切片。该方法为从解剖学上研究林木茎段生长机制和形态发育变化提供技术基础,为非均质化植物材料的石蜡切片制作提供参考。
In view of the characteristics of highly lignified, large hardness and heterogeneous of various parts of woody plants, the stem segments and healing areas of bud grafting of Juglans regia were used as materials with the conven- tional method of paraffin sectioning. The aspects of fixation, softening, dehydration and other critical steps were modified to obtain cross-section slices with complete organizational structure and clear staining. The morphological and develop- mental characteristics of the healing period could be observed clearly and shortened the experimental period. The paraffin sections of stem segments of apple, peach, apricot, plum and poplar were obtained by the improved experimental proc- ess. The study provides a technical basis for studying the growth mechanism and morphological development of stem segments of woody plants and provides a reference for making paraffin sections of heterogeneous plant materials.