文献类型：期刊文献

英文题名：3-ketoacyl-CoA synthase 7 from Xanthoceras sorbifolium seeds is a crucial regulatory enzyme for nervonic acid biosynthesis

作者：Li, Linkun[1] Liang, Chongjun[1] Zhang, Wei[1] Zhang, Xin[2] Yu, Haiyan[1] Liu, Xiaojuan[1] Bi, Quanxin[1] Wang, Libing[1,2]

第一作者：Li, Linkun

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Forestry, Yangling 712100, Peoples R China

年份：2024

卷号：347

外文期刊名：PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85198499698);WOS:【SCI-EXPANDED(收录号:WOS:001272518700001)】；

基金：We thank National Youth Talent Support Program of China and National Natural Science Foundation of China (Grant No. 31870594) for financial support. We also like to thank the reviewers for positive crit- icism to improve the quality of the manuscript.

语种：英文

外文关键词：3-ketoacyl-CoA synthase; Regulatory enzyme; Nervonic acid; Gene functional identification; Xanthoceras sorbifolium seed

摘要：Nervonic acid (C24:1) is a very-long-chain fatty acid that plays an imperative role in human brain development and other health benefits. In plants, 3-ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for C24:1 biosynthesis. Xanthoceras sorbifolium is a valuable oil-producing economic woody species with abundant C24:1 in seed oils, but the key KCS gene responsible for C24:1 accumulation remains unknown. In this work, a correlation analysis between the transcript profiles of KCS and dynamic change of C24:1 content in developing seeds of X. sorbifolium were conducted to screen out three members of KCS, namely XsKCS4, XsKCS7 and XsKCS8, potentially involved in C24:1 biosynthesis. Of which, the XsKCS7 was highly expressed in developing seeds, while XsKCS4 and XsKCS8 displayed the highest expression in fruits and flowers, respectively. Overexpression of XsKCS4, XsKCS7 and XsKCS8 in yeast Saccharomyces cerevisiae and plant Arabidopsis thaliana indicated that only XsKCS7 possessed the ability to facilitate the biosynthesis of C24:1. These findings collectively suggested that XsKCS7 played a crucial role in specific regulation of C24:1 biosynthesis in X. sorbifolium seeds.