文献类型：期刊文献

中文题名：撕裂耙齿菌生物学特性及利用竹屑驯化栽培

英文题名：Biological characteristics of Irpex laceratus and domesticated cultivation using bamboo shavings

作者：李东晨[1] 张望舒[2] 茆卫琳[1] 张磊[1,3] 张健[4] 王保玺[1] 刘晓草[1] 陈胜[1] 谢锦忠[1] 王欣[1]

第一作者：李东晨

机构：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400;[2]浙江大学生物系统工程与食品科学学院,浙江杭州310058;[3]忠县林业局,重庆404300;[4]安吉县林业局,浙江安吉313300

年份：2025

卷号：44

期号：11

起止页码：194-209

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：;北大核心:【北大核心2023】；

基金：2022安吉县绿色竹产业创新服务综合体重点研发项目;宁波市科技计划项目(2019B10020);科技对口支援和东西部协作项目(2022C04013);浙江省省院合作林业科技项目(2020SY15)。

语种：中文

中文关键词：药用真菌;正交试验;毛竹竹屑;人工栽培;活性物质

外文关键词：medicinal fungi;orthogonal experiment;Moso bamboo chips;artificial cultivation;active substance

分类号：S567.3

摘要：以一株分离自玉木耳Auricularia cornea菌包的耙齿菌Irpex菌株为研究对象,通过形态学观察、ITS测序分析、生物学特性研究、栽培试验进行鉴定和驯化。形态学和分子生物学鉴定表明,该菌株为撕裂耙齿菌Irpex laceratus;正交试验结果表明,影响撕裂耙齿菌菌丝生长主要因素为氮源,其次为碳源、pH和温度。栽培驯化表明,撕裂耙齿菌最佳碳氮比为50:1–52:1,添加14.5%–43.5%竹屑可加快菌丝生长速度,提高菌丝长势;不同配方活性物质有所差异,配方B(木屑29%,竹屑29%,麦麸20%,棉籽壳20%,石灰1%,石膏1%)总三萜含量最大,为66.10 mg/g;配方I(竹屑78%,麦麸20%,石灰1%,石膏1%)总蛋白含量最高,为21.83 g/100 g;配方CK(木屑58%,麦麸20%,棉籽壳20%,石灰1%,石膏1%)总黄酮含量最高,为0.99 mg/g;配方C(木屑14.5%,竹屑43.5%,麦麸20%,棉籽壳20%,石灰1%,石膏1%)多糖含量最高,为2.80 g/100 g;配方F(竹屑68%,麦麸20%,棉籽壳10%,石灰1%,石膏1%)总酚含量最高,为19.02 mg/g。研究结果为撕裂耙齿菌栽培和药物开发利用提供了参考。
A strain of Irpex was isolated from Auricularia cornea-cultivated substrate using banboo shavings.Through morphological observation and ITS sequencing analysis,the strain was identified as Irpex laceratus.The orthogonal experiment results indicated that the main factor affecting the mycelial growth of I.laceratus was the nitrogen source,followed by the carbon source,pH and temperature.The optimal carbon to nitrogen ratio is 50:1–52:1.Addition of 14.5%–43.5%bamboo shavings significantly accelerated mycelial growth.The content of active substances varied with different formulations of cultivated substrates.Formula B(wood shavings 29%,bamboo shavings 29%,wheat bran 20%,cottonseed hulls 20%,lime 1%,gypsum 1%)exhibited the highest total triterpene content(66.10 mg/g);formula I(bamboo shavings 78%,wheat bran 20%,lime 1%,gypsum 1%)showed the highest total protein content(21.83%);formula CK(wood chips 58%,wheat bran 20%,cottonseed husks 20%,lime 1%,gypsum 1%)exhibited the highest total flavonoid content(0.99 mg/g);formula C(wood chips 14.5%,bamboo chips 43.5%,wheat bran 20%,cottonseed husks 20%,lime 1%,gypsum 1%)had the highest polysaccharide content of 2.80 g/100 g;formula F(bamboo chips 68%,wheat bran 20%,cottonseed husks 10%,lime 1%,gypsum 1%)demonstrated the highest total phenol content of 19.02 mg/g.The results provide valuable reference data for the cultivation and medicinal development of I.laceratus.